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Merck
모든 사진(1)

주요 문서

T2507

Sigma-Aldrich

Triosephosphate Isomerase from baker′s yeast (S. cerevisiae)

Type I, ammonium sulfate suspension, ~10,000 units/mg protein

동의어(들):

D-Glyceraldehyde-3-phosphate ketol-isomerase, TPI

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About This Item

CAS Number:
효소 위원회 번호:
EC Number:
MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.54

유형

Type I

Quality Level

양식

ammonium sulfate suspension

특이 활성도

~10,000 units/mg protein

분자량

53 kDa by equilibrium centrifugation

외래 활성

pyruvate kinase, lactic dehydrogenase, 3-phosphoglyceric phosphokinase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase <0.01%

저장 온도

2-8°C

애플리케이션

Triosephosphate isomerase has been used in a study to assess differential expression of fourteen proteins of uveal melanoma. Triosephosphate isomerase has also been used in a study to investigate the use of sigmoid pH gradients in free-flow isoelectric focusing of human endothelial cell proteins.

단위 정의

One unit will convert 1.0 μmole D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C.

물리적 형태

Crystalline suspension in 2.7 M (NH4)2SO4, 0.5 mM EDTA, pH 6.5

분석 메모

Protein determined by biuret.

기타 정보

Since the assay of this enzyme seems to be sensitive to minor variables, packages are dispensed per mg protein rather than in units.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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시험 성적서(COA)

Lot/Batch Number

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문서 라이브러리 방문

Annett Linge et al.
Investigative ophthalmology & visual science, 53(8), 4634-4643 (2012-05-10)
To compare the proteomic profiles of two categories of primary uveal melanoma tissue samples; those from patients who have subsequently developed metastatic disease and those who have not. Two-dimensional difference gel electrophoresis (2D DIGE) was performed on 25 uveal melanoma
Xiao Ma et al.
Electrophoresis, 33(9-10), 1349-1355 (2012-06-01)
Prefractionation of proteins enhances the resolution of proteome analysis of whole cells. Free-flow electrophoresis (FFE) provides a useful step in various prefractionation protocols, since matrix-free isoelectric focusing (FF-IEF) performed in this machine enables the enrichment of large, easily absorbable, sensitive
Matthew J Nolan et al.
Water research, 47(5), 1726-1740 (2013-01-30)
There has been no large-scale systematic molecular epidemiological investigation of the waterborne protozoans, Cryptosporidium or Giardia, in southeastern Australia. Here, we explored, for the first time, the genetic composition of these genera in faecal samples from animals in nine Melbourne
Jessica Pietsch et al.
Proteomics, 12(15-16), 2539-2546 (2012-06-19)
Many cancer cells show unique protein expression patterns. We used proteome technology including MS, free flow isoelectric focusing and Western blotting to determine current concentrations of metabolic enzymes in healthy and malignant human thyroid cells. Three different types of human
Rongchang Yang et al.
Experimental parasitology, 137, 46-52 (2013-12-21)
A novel quantitative PCR (qPCR) for Giardia at the glutamate dehydrogenase (gdh) locus was developed and validated. The qPCR was used to screen a total of 3412 lamb faecal samples collected from approximately 1189 lambs at three sampling periods (weaning

문서

Instructions for working with enzymes supplied as ammonium sulfate suspensions

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