SRP6414

Cathepsin B human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• 동의어(들): APPS, CPSB, CTSB
• UNSPSC 코드: 12352200
• NACRES: NA.32

속성

• 생물학적 소스: human
• 재조합: expressed in HEK 293 cells
• 태그: 6-His tagged (C-terminus)
• 분석: ≥95% (SDS-PAGE)
• 형태: lyophilized
• 분자량: calculated mol wt 29 kDa (Mature); calculated mol wt 36.7 kDa (Pro); observed mol wt 34 kDa; observed mol wt 43_kDaand34 kDa
• 포장: pkg of 10 μg
• 불순물: <1 EU/μg endotoxin (LAL test)
• UniProt 수납 번호: P07858
• 배송 상태: wet ice
• 저장 온도: −20°C
• 유전자 정보: human ... CTSB(1508)

설명

일반 설명

Cathepsin B (CTSB) also known as APP secretase (APPS) and CPSB, is an enzymatic protein belonging to the peptidase C1 family. Cathepsin B / CTSB is synthesized as a preproenzyme. Following removal of the signal peptide, the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme. The catalytic activity of Cathepsin B / APPS contains: Hydrolysis of proteins with broad specificity for peptide bonds; preferentially cleaves -Arg-Arg-|-Xaa bonds in small molecule substrates (thus differing from cathepsin L); In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides. As a thiol protease, cathepsin B / CPSB is believed to participate in intracellular degradation and turnover of proteins and has also been implicated in tumor invasion and metastasis. Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors.

DTT-reduced. Protein migrates due to glycosylation. Arg18 (pro) or Phe74 (mature) is the predicted N-terminal.

물리적 형태

Lyophilized from 0.22 μm filtered solution in 50 mM Tris-HCl and 150 mM NaCl, pH 8.0. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

재구성

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable