SRP6267

C-reactive/CRP human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• 동의어(들): C-reactive protein, CRP, PTX1
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 생물학적 소스: human
• 재조합: expressed in HEK 293 cells
• 태그: 6-His tagged (C-terminus)
• 분석: ≥95% (SDS-PAGE)
• 형태: lyophilized powder
• 분자량: calculated mol wt 24 kDa; observed mol wt 26 kDa (DTT-reduced. Phe17 is the predicted N-terminus.)
• 포장: pkg of 100 μg
• 불순물: <1 EU/μg endotoxin (LAL test)
• UniProt 수납 번호: P02741
• 배송 상태: wet ice
• 저장 온도: −20°C
• 유전자 정보: human ... CRP(1401)

설명

일반 설명

C-reactive protein (CRP) is a member of the pentraxin family of proteins that are characterized by a cyclic pentameric structure. Human CRP gene encodes a 224 amino acids precursor. The mature human CRP protein has 206 amino acids that are noncovalently linked to form the pentameter. Human CRP shares 71% and 64% amino acid sequence homology with mouse and rat, respectively.

생화학적/생리학적 작용

CRP, synthesized by hepatocytes, is a major acute phase serum protein in human. IL6, IL1 and glucocorticoids are the major inducer of the CRP gene. The physiological role of CRP is to bind to phosphocholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system. CRP binds to phosphocholine on microbes and damaged cells and enhances phagocytosis by macrophages. Thus, CRP participates in the clearance of necrotic and apoptotic cells. It has been shown that high levels of CRP in humans is associated with an increased risk of cardiovascular diseases.

물리적 형태

Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

재구성

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable