SRP5148

Tubulin, GST tagged human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• 동의어(들): M40, MGC117247, MGC16435, OK/SW-cl.56, TUBB, TUBB5
• CAS Number: 1622402-26-9
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 생물학적 소스: human
• 재조합: expressed in E. coli
• 분석: ≥70% (SDS-PAGE)
• 양식: buffered aqueous glycerol solution
• 분자량: ~76 kDa
• NCBI 수납 번호: NM_178014
• 응용 분야: cell analysis
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... TUBB(203068)

설명

일반 설명

Tubulin-β 1 (TUBB1) protein is a major constituent of microtubules. Previous work suggested that the "microtubule binding domain" of tau (composed of three or four imperfect 18-amino acid repeats, separated by 13- or 14-amino acid inter-repeat regions) can bind to the C-terminal ends of both α and β tubulin monomers. It is found in hematopoietic cells and platelet marginal bands. The gene encoding TUBB1 is localized on human chromosome 6p21.

애플리케이션

GST tagged tubulin has been used in Western blotting and in mass spec as a control.

생화학적/생리학적 작용

Tubulin-β 1 (TUBB1) interaction with microtubule-associated proteins (MAPs) such as tau is fundamental for microtubule structure and function. It has a role in the formation of the cytoskeleton. Studies revealed that TUBB1 is the target of various antitubulin agents used in the treatment of cancer. Subsequent studies have also concluded that TUBB1 mutations in clinical samples are rare, and unlikely to contribute to drug resistance.

물리적 형태

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

제조 메모

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable