SRP3285

GREMLIN-1 human

recombinant, expressed in CHO cells, ≥90% (SDS-PAGE), ≥90% (HPLC)

• 동의어(들): CKTSF1B1, DAND2, DRM
• UNSPSC 코드: 12352200
• NACRES: NA.32

속성

• 생물학적 소스: human
• 재조합: expressed in CHO cells
• 분석: ≥90% (HPLC); ≥90% (SDS-PAGE)
• 형태: lyophilized
• 효능: 0.07-0.11 μg/mL
• 분자량: 13.7 kDa
• 포장: pkg of 50 μg
• 불순물: <0.1 EU/μg endotoxin, tested
• 색상: white to off-white
• UniProt 수납 번호: O60565
• 배송 상태: wet ice
• 저장 온도: −20°C
• 유전자 정보: human ... GREM1(26585)

설명

일반 설명

Gremlin-1 (isoform-1) belongs to a group of diffusible proteins which bind to ligands of the transforming growth factor-β (TGF-β) family and regulate their activity by inhibiting their access to signaling receptors. The gene encoding this protein is localized on human chromosome 15q13.3. Recombinant Gremlin-1 is a 13.7kDa protein containing 160 amino acid residues.

생화학적/생리학적 작용

The interplay between transforming growth factor-β (TGF-β) ligands and their natural antagonists has major biological significance during development processes, in which cellular response can vary considerably depending upon the local concentration of the signaling molecule. Gremlin is highly expressed in the small intestine, fetal brain, and colon and lower expression in brain, prostate, pancreas and skeletal muscle. Gremlin-1 regulates multiple functions in early development by specifically binding to and inhibiting the function of bone morphogenetic proteins -2,-4,-7 (BMP-2, -4, and -7). The protein has a role in limb and digit formation and in nephrogenesis. It also plays a role in carcinogenesis and kidney branching morphogenesis.

물리적 형태

Lyophilized from 10 mM Sodium Phosphate, pH 7.5 + 150 mM NaCl.

재구성

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable