SRP3109

MCP-1/MCAF human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• 동의어(들): CCL2, MCAF, Monocyte Chemotactic Protein-1
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 생물학적 소스: human
• 재조합: expressed in E. coli
• 분석: ≥98% (HPLC); ≥98% (SDS-PAGE)
• 형태: lyophilized
• 분자량: 8.6 kDa
• 포장: pkg of 20 μg
• 기술: cell culture | mammalian: suitable
• 불순물: <0.1 EU/μg endotoxin, tested
• 색상: white to off-white
• UniProt 수납 번호: P13500
• 배송 상태: wet ice
• 저장 온도: −20°C
• 유전자 정보: human ... CCL2(6347)

설명

일반 설명

Monocyte chemoattractant protein-1/monocyte chemotactic and activating factor (MCP-1/MCAF), also called the C-C motif chemokine ligand 2 (CCL2), is an inflammatory cytokine. The CCL2 gene is mapped to human chromosome 17q12. CCL2 is present in epithelial, endothelial, mesangial and microglial cells. Structurally, it dimerizes and has four regions of the ß-sheet. Recombinant human MCP-1 is a 8.6 kDa protein containing 76 amino acid residues including the four highly conserved cysteine residues. The MCP family cross-reacts across species.

애플리케이션

Monocyte chemoattractant protein-1/monocyte chemotactic and activating factor (MCP-1/MCAF) has been used:

• as a chemokine supplement in the alpha minimal essential medium for culturing neurons to test its effect on JmjC histone demethylase (Jmjd2a) mRNA
• as a component of Dulbecco′s Modified Eagle (DMEM) medium, as a positive chemoattractant control in HeLa cells for migration assay
• as a chemoattractant to induced macrophage chemotaxis

생화학적/생리학적 작용

Monocyte chemoattractant protein-1/monocyte chemotactic and activating factor (MCP-1/MCAF) acts as a human immunodeficiency virus 1 (HIV-1) suppressor. The MCP-1 acts as a chemoattractant for T cells and natural killer (NK) cells. It also plays a key role in the migration and infiltration of macrophages. CCL2 shows under expression in high-grade ovarian serous carcinomas (HGOSCs) and is expressed less in epithelial ovarian cancer (EOC).

Monocyte chemoattractant protein-1/monocyte chemotactic and activating factor (MCP-1/MCAF) is a member of CC chemokine family. It triggers the activation of monocytes by triggering degranulation as well as respiratory burst activity in monocytes. It facilitates monocytes in inhibiting or enhancing the growth of specific brain tumor cell lines. It is found to be overexpressed in cases of atherosclerosis, idiopathic pulmonary fibrosis, rheumatoid arthritis, and delayed hypersensitivity reactions. It is also implicated in Kawasaki disease.

서열

QPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT

물리적 형태

Lyophilized with no additives.

재구성

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable