SRP3045

Follistatin human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• 동의어(들): FS, activins-binding protein
• UNSPSC 코드: 12352200
• NACRES: NA.32

속성

• 생물학적 소스: human
• 재조합: expressed in E. coli
• 분석: ≥98% (HPLC); ≥98% (SDS-PAGE)
• 형태: lyophilized
• 효능: 0.1-0.4 μg/mL
• 분자량: 31.5 kDa
• 포장: pkg of 20 μg
• 기술: cell culture | mammalian: suitable
• 불순물: <1.0 EU/μg endotoxin, tested
• 색상: white to off-white
• UniProt 수납 번호: P19883
• 배송 상태: wet ice
• 저장 온도: −20°C
• 유전자 정보: human ... FST(10468)

설명

일반 설명

Follistatin is a secreted protein that binds to ligands of the TGF (transforming growth factor)-β family and regulates their activity by inhibiting their access to signaling receptors. Follistatin is expressed in the pituitary, ovaries, decidual cells of the endometrium, and in some other tissues. The gene is mapped to human chromosome 5q11.2. Recombinant human Follistatin is a 31.5kDa protein containing 288 amino acids. Its primary structure contains three cysteine-rich domains (called FS domains), each followed by a protease-inhibitory kazal domain.

애플리케이션

Follistatin human has been used to suppress myostatin signaling. It has also been used as an inhibitor of activin A.

생화학적/생리학적 작용

Follistatin is a multifunctional protein. It was originally discovered as activin antagonists whose activity suppresses expression and secretion of the pituitary hormone FSH (follicle stimulating hormone). In addition to being a natural antagonist, follistatin can inhibit the activity of other TGF (transforming growth factor)-β ligands including BMP (bone morphogenetic protein)-2,-4,-6,-7, Myostatin, GDF-11 (growth differentiation factor 11), and TGF-b1. In gonads, it is responsible for oocyte competence and embryo development. In the pituitary gland, it causes cyclic changes in FSH production in the estrous cycle. Follistatin also participates in bone metabolism. It controls inflammation, fibrosis and tissue repair. Levels of plasma follistatin increases in polycystic ovary syndrome and type 2 diabetes. Follistatin might also be involved in tumor progression, including apoptosis, angiogenesis, and metastasis.

서열

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물리적 형태

Lyophilized from 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.

재구성

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H315 - H319 - H335
• 예방조치 성명서: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable