SRP2088

C-jun, proto oncogene human

recombinant, expressed in insect cells, ≥80% (SDS-PAGE)

• 동의어(들): AP-1, AP1, c-Jun
• UNSPSC 코드: 12352200
• NACRES: NA.26

속성

• 생물학적 소스: human
• 재조합: expressed in insect cells
• 분석: ≥80% (SDS-PAGE)
• 형태: frozen liquid
• 분자량: ~37.5 kDa
• 포장: pkg of 5 μg
• 저장 조건: avoid repeated freeze/thaw cycles
• 농도: 250 μg/mL
• 색상: clear; colorless
• NCBI 수납 번호: NM_002228
• UniProt 수납 번호: P05412
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... JUN(3725)

설명

생화학적/생리학적 작용

The transcription factor AP-1 (activator protein-1) is involved in cellular proliferation, transformation and death. AP-1 and nuclear factor B (NF- B) can be specifically targeted to prevent cancer induction in mouse models. AP-1 can be produced by 18 different dimeric combinations of proteins from the Jun (c-Jun, JunB and JunD) and Fos (c-Fos, FosB, Fra-1 and Fra-2) families, including Jun homodimers and Jun-Fos heterodimers. The Jun and Fos proteins contain a basic-region leucine zipper (bZIP) domain, and are capable of binding to other bZIP proteins including those from the ATF, MAF, CNC and C/EBP (CCAAT/enhancer-binding protein) subfamilies (5]) Jun-Jun and Jun-Fos dimers bind with highest affinity to the 12-O-tetradecanoylphorbol-13-acetate (TPA) response element (TRE) [TGA(C/G)TCA], although many other ′AP-1-like sites′ have been reported. Binding to any of these sites can be tissue-specific, or affected by neighboring sequences, and dependent upon interactions with other transcription factors or cofactors. Jun and Fos proteins can also dimerize with other bZIP proteins, allowing them to target other DNA binding sites, such as the cAMP response element (CRE), the antioxidant response elements (ARE), and half-sites composed of half of a TRE site and half of a MAF- or CNC-binding site. In addition, AP-1 proteins can interact with other proteins, including the p65 subunit of NF- B, CBP (CRE-binding-protein-binding protein) (p300), SMAD-3 and -4, and the retinoblastoma protein (see 5 for a more complete list), further increasing the combinatorial potential of Jun and Fos proteins. AP-1 regulates a variety of cellular processes, including proliferation, differentiation and apoptosis, and contributes to both basal and stimulus-activated gene expression. It is activated by growth factors, hormones, stress, cytokines, ROS and ultraviolet radiation Activation occurs both transcriptionally and post-translationally, and is signaled predominantly through the mitogen-activated protein kinase (MAPK) cascade. The combinatorial diversity of AP-1 proteins and other interacting factors appears to influence how specific cell types respond to a stimulus. The growth-promoting activity of c-Jun is mediated by repression of tumor suppressors, as well as up-regulation of positive cell cycle regulators. Mostly, c-Jun is a positive regulator of cell proliferation, whereas JunB has the converse effect.

물리적 형태

Clear and colorless frozen liquid solution

제조 메모

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable