SRP0721

AKT1 (E17K), Active

PRECISIO^® Kinase, recombinant

• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• 재조합: expressed in baculovirus infected Sf9 cells
• 분석: 70-95% (SDS-PAGE)
• 형태: frozen liquid
• 특이 활성도: 50-75 nmol/min·mg (radiometric)
• 분자량: ~85 kDa
• 농도: 0.1 μg/μL
• 수납 번호(accession number): NM_005163
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... AKT1(207)

설명

애플리케이션

Useful for kinase assay.

생화학적/생리학적 작용

Recombinant full-length human AKT1 (E17K) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. AKT1/PKBalpha is a serine/threonine kinase that belongs to the AKT family. AKT1 is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility ⁽¹⁾. AKT1 is frequently overexpressed and active in many types of human cancers including cancers of colon, breast, brain, pancreas and prostate as well as lymphomas and leukemias ⁽²⁾.

재구성

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

저장 및 안정성

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

법적 정보

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable