SRP0691

PI3K (P85 ALPHA) Protein

recombinant

• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• 재조합: expressed in baculovirus infected Sf9 cells
• 분석: 70-95% (SDS-PAGE)
• 형태: frozen liquid
• 분자량: ~86 kDa
• 농도: 0.2 μg/μL
• 수납 번호(accession number): NM_181523
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... PI3K (5295)

설명

애플리케이션

Useful for Western blot and kinase assays.

생화학적/생리학적 작용

Recombinant full-length human PI3K (p85alpha) was expressed by Baculovirus in Sf9 insect cells using an N-terminal His tag. The PI3K comprises of a 110 kDa catalytic subunit and a 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. p85α modulates the interaction between PI3K and platelet-derived growth factor receptor ⁽¹⁾. Furthermore, estrogen receptor isoform ER-alpha binds in a ligand-dependent manner to the p85-alpha regulatory subunit of PI3K. Stimulation with estrogen increases ER-alpha-associated PI3K activity, leading to the activation of protein kinase B/AKT and endothelial nitric oxide synthase (eNOS) ⁽²⁾.

재구성

Formulated in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, with 25% glycerol.

저장 및 안정성

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

안전성 정보

• 픽토그램: GHS08,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H315,H319,H360D
• 예방조치 성명서: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable