SRP0222

PTPmu Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• 동의어(들): PTPRL1, PTPRM, Protein tyrosine phosphatase mu, R-PTP-MU, hR-PTPu
• UNSPSC 코드: 12352200
• NACRES: NA.32

속성

• 생물학적 소스: human
• 재조합: expressed in E. coli
• 분석: ≥90% (SDS-PAGE)
• 형태: aqueous solution
• 분자량: 56.2 kDa
• 포장: pkg of 20 μg
• 저장 조건: avoid repeated freeze/thaw cycles
• 농도: >0.02 mg/mL
• NCBI 수납 번호: BC051651
• UniProt 수납 번호: P28827
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... PTPRM(5797)

설명

일반 설명

Protein tyrosine phosphatase. Catalytic domain, (amino acids 839-1100), (Gene bank accession number BC051651) with N-terminal GST tag, MW = 56.2 kDa, expressed in an E. coli expression system.

애플리케이션

Useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates.

단위 정의

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

물리적 형태

Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.

제조 메모

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable