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Merck
모든 사진(1)

Key Documents

SRP0128

Sigma-Aldrich

DNMT3a Active human

recombinant, expressed in baculovirus infected insect cells

동의어(들):

DNA (cytosine-5-)-methyltransferase 3A, DNA MTase HsaIIIA, DNMT3A2, M.HsaIIIA

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About This Item

UNSPSC 코드:
12352200
NACRES:
NA.32

생물학적 소스

human

재조합

expressed in baculovirus infected insect cells

형태

aqueous solution

분자량

128 kDa

포장

pkg of 10 μg

농도

>0.02 mg/mL

UniProt 수납 번호

배송 상태

dry ice

저장 온도

−70°C

유전자 정보

human ... DNMT3A(1788)

일반 설명

DNMT3A (DNA methyltransferase 3α) is one of three (DNMT1, DNMT3a and DNMT3b) active forms of DNA methyltransferases. This gene is localized to human chromosome 2p23. DNMT3A is one of the three members of the DNMT3A family, of which DNMT3A and DNMT3B are catalytically active and DNMT3L is an inactive paralogue, which has regulatory actions in germ cells.

애플리케이션

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

생화학적/생리학적 작용

DNA methyltransferases are responsible for the addition of a methyl residue to the cytosine group of CpG dinucleotides. DNMT3A (DNA methyltransferase 3α) participates in the gene expression suppression by interacting with histone modifiers, such as polycomb-group (PcG). The interaction between these two proteins is thought to be implicated in cancer pathogenesis. During embryogenesis this enzyme is responsible for the formation of genomic methylation patterns and germ line development. Polymorphisms in this gene might serve as biomarkers to determine the prognosis of gastric cancer patients. Mutations in this gene are linked with Tatton-Brown-Rahman syndrome, an overgrowth syndrome characterized by unique facial appearance, intellectual disability, and increased height.

물리적 형태

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 10% glycerol.

제조 메모

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

DNMT3A mutations in acute myeloid leukemia.
Ley TJ et al
The New England Journal of Medicine, 363(25), 2424-2433 (2010)
DNA methyltransferase 3a rs1550117 genetic polymorphism predicts poor survival in gastric cancer patients.
Wang C et al
International Journal of Clinical and Experimental Pathology, 8(11), 14864-14874 (2015)
DNMT3A R882 mutants interact with polycomb proteins to block haematopoietic stem and leukaemic cell differentiation.
Koya J et al
Nature Communications, 7, 10924-10924 (2016)
Tatton-Brown-Rahman syndrome due to 2p23 microdeletion.
Okamoto N et al
American Journal of Medical Genetics. Part A, 170A(5), 1339-1342 (2016)
Structure of Dnmt3a bound to Dnmt3L suggests a model for de novo DNA methylation.
Jia D et al
Nature, 449(7159), 248-251 (2007)

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