SRE0023

β-Glucuronidase from abalone

Purified, aqueous solution, β-glucuronidase 150,000-250,000 units/mL, β-glucuronidase ≥20,000,000 units/g protein

• 효소 위원회 번호: 3.1.2.31 (BRENDA, IUBMB)
• MDL number: MFCD00130629
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• Quality Level: 200
• 양식: aqueous solution
• β-glucuronidase activity: ≥20,000,000 units/g protein; 150,000-250,000 units/mL
• secondary activity: ≤7,500 units/mL sulfatase
• 저장 온도: 2-8°C

설명

애플리케이션

Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine.

생화학적/생리학적 작용

β-Glucuronidase (GUSB) is a lysosomal enzyme, that degrades glucuronate-containing glycosaminoglycans. β-glucuronidase, obtained from abalone has the ability to hydrolyze samples, that has high analyte concentrations. Absence of β-glucuronidase results in mucopolysaccharidosis type VII (MPSVII), which leads to lysosomal storage in the brain.

단위 정의

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay).

물리적 형태

Supplied as an aqueous solution containing L-proline, ammonium acetate and sodium azide

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable