G0251

β-Glucuronidase from bovine liver

Type B-1, ≥1,000,000 units/g solid

• 동의어(들): Bovine Liver beta-Glucuronidase, β-D-Glucuronide glucuronosohydrolase
• CAS Number: 9001-45-0
• 효소 위원회 번호: 3.2.1.31 (BRENDA, IUBMB)
• EC Number: 232-606-8
• MDL number: MFCD00130629
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 유형: Type B-1
• 형태: solid
• 특이 활성도: ≥1,000,000 units/g solid
• 저장 온도: −20°C

설명

일반 설명

β-Glucuronidase (GUSB) is a lysosomal enzyme, found in animals, plants and bacteria. This exoglycosidase shows high activity in spleen, liver and kidney. Bovine liver β-glucuronidase (BLG) is homotetramer with a molecular weight of 290 kDa.

애플리케이션

β-Glucuronidase from bovine liver has been used in β-glucuronidase inhibition assay to study the β-glucuronidase inhibitory effect of extracts and isolated actives from Phyllanthus amarus. It has also been used to perform deglucuronidation.

β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.

생화학적/생리학적 작용

β-Glucuronidase (GUSB) deficiency can result in mucopolysaccharidosis type VII (MPSVII), which leads to store lysosome in the brain. Integration of β-glucuronides of steroid hormones into tissues can be done with the help of GUSB.

β-glucuronidase catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.

단위 정의

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H317,H334
• 예방조치 성명서: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)