SML1154

Clostridium difficile Toxin A

≥95% (SDS-PAGE), lyophilized powder, cellular toxin

• 동의어(들): TcdA
• MDL number: MFCD02098001
• UNSPSC 코드: 12352200
• NACRES: NA.77

속성

• product name: Clostridium difficile Toxin A, ≥95% (SDS-PAGE)
• Quality Level: 200
• 분석: ≥95% (SDS-PAGE)
• 형태: lyophilized powder
• 저장 온도: 2-8°C

설명

일반 설명

Native toxin A purified from C. difficile strain VPI10463 (toxinotype 0)

생화학적/생리학적 작용

Clostridium difficile Toxin A and B, cation-dependent UDP-glucose glucosyltransferases, are cellular toxins that inactivate Rho (and Rho family small GTPases) through monoglucosylation of these family members. TcdA elicits effects primarily within the intestinal epithelium. Effects of this monoglucosylation include disregulation of the actin cytoskeleton, cell rounding, cytotoxicity, and altered cellular signaling. Rho proteins are monoglucosylated by Toxin A and B using UDP-glucose as a cosubstrate. Rho, Rac and Cdc42 are included in the Rho subfamilies targeted by both toxins. Low molecular mass GTP-binding proteins that are not modified by Toxin A and B include Ras, Rab, Arf, or Ran subfamilies as well as heterotrimeric G proteins.

재구성

Contents of the 50UG sku when reconstituted in 250 μL sterile distilled water will contain Toxin A at a concentration of approximately 0.2 mg/mL in 0.05M Hepes, 0.15M sodium chloride and 5% sucrose.
Contents of the 2UG sku when reconstituted in 250 μL sterile distilled water will contain Toxin A at a concentration of approximately 8 μg/mL in 0.05M Hepes, 0.15M sodium chloride and 5% sucrose.

기타 정보

After reconstitution, the reconstituted liquid can be frozen at -80 °C to -20 °C to extend stability to 1 year; however every freeze/thaw cycle wil cause increased aggregation. Avoid multiple freeze/thaw cycles and multiple temperature fluctuations.
Mixing: Swirl or pipette gently to mix. Vortexing may promote aggregation.

안전성 정보

• 픽토그램: GHS06
• 신호어: Danger
• 유해 및 위험 성명서: H301,H312 + H332
• 예방조치 성명서: P261 - P264 - P280 - P301 + P310 - P302 + P352 + P312 - P304 + P340 + P312
• Hazard Classifications: Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable