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Merck
모든 사진(2)

Key Documents

SCT089

Sigma-Aldrich

BioTracker rRNA Live Cell Probe

Fluorescent detection of ribosomal RNA and cell nucleoli

동의어(들):

live cell probe, nucleoli probe, nucleolus probe, rRNA dye, rRNA probe, ribosomal RNA probe

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About This Item

UNSPSC 코드:
12171501
NACRES:
NA.31

Quality Level

형태

solid

배송 상태

ambient

저장 온도

2-8°C

일반 설명

The BioTrackerrRNA live cell probe is a styryl quinolinium dye that selectively binds ribosomal RNA (rRNA) in the cytoplasm and nucleoli of mammalian cells 1. This probe is a fluorescent small molecule that demonstrates high affinity to rRNA and therefore enables imaging of the nucleolus. Due to its low cytotoxicity, it enables detection in living systems with minimal interference.1 . As imaging technologies progress, there is increased demand for reagents that can detect diverse organoids and enable observation of subcellular functions in living cells. The optimal fluorescent reagent for live cell imaging should show high specificity to target along with low cytotoxicity 2 . Because the nucleolus is a dense organoid composed of chromatin and numerous protein components, the development of fluorescent reagents for live cell nucleoli detection is even more challenging 2,3,4. BioTracker rRNA live cell probe is easy to use, emits light in a longer wavelength to reduce phototoxicity and facilitate multiplexing, and can enhance nucleolar imaging dynamics.

Spectral Properties:Fluorescence images obtained by λex = 570 nm and emission at 647-667 nm.

애플리케이션

Live cell imaging probefor detection of ribosomal RNA (rRNA). Can also be used in fixed cell or tissuesamples, such as with a nuclear counterstain to identify nucleoli.

품질

Purity: ≥ 95% confirmed by HPLC. Molar Mass: 298 Da confirmed byLC-MS.

저장 및 안정성

Store BioTracker rRNA Live CellProbe at 2-8 °C, desiccated and protected from light

Note: Centrifuge vial briefly to collect contents at bottom ofvial before opening.

법적 정보

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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