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일반 설명
SOLVO Biotechnology offers ready-to-use assay kits for efflux and uptake transporters. SOLVO′s PREDEASY™ ATPase Assay Kit provides everything necessary to conduct ATPase activation and inhibition assays to better understand the nature of the interaction between a compound and the transporter. By measuring ATPase activity, both activation and inhibition of transporters can be investigated.
애플리케이션
SOLVO′s PREDEASY™ ATPase Assay Kit is a unique product for measuring the ATPase-dependent drug - ABC membrane transporter interaction in a convenient, "ready-to-assay" package. The ATPase assay is an in vitro membrane assay designed to indicate the nature of the interaction between the compound and the transporter. By measuring ATPase activity, both activation and inhibition of transporters can be investigated using membranes from baculovirus-infected insect cells or mammalian cell membranes containing high levels of human or rodent wild-type transporters. ABC transporters mediate the transport of substrates against a concentration gradient using energy derived from ATP hydrolysis, which is proportional to the transporter activity and could easily be detected with a colorimetric method.
PREDIVEZ™ PREDEASY™ Kits contain all reagents (chemicals) and solutions necessary to conduct ATPase activation and inhibition assays. You only have to add water. Each kit comes with a CD that includes the assay protocol, data sheet, SDS, electronic data processing, evaluation templates and the reference data. The recommended layout allows activation and inhibition measurements in duplicates for 8 concentrations of the test article on one 96-well plate with all of the relevant controls.
PREDEASY™ ATPase Assay Kits are available for 3 and 10 plates for the transporters recommended by the FDA and the EMA.
PREDIVEZ™ PREDEASY™ Kits contain all reagents (chemicals) and solutions necessary to conduct ATPase activation and inhibition assays. You only have to add water. Each kit comes with a CD that includes the assay protocol, data sheet, SDS, electronic data processing, evaluation templates and the reference data. The recommended layout allows activation and inhibition measurements in duplicates for 8 concentrations of the test article on one 96-well plate with all of the relevant controls.
PREDEASY™ ATPase Assay Kits are available for 3 and 10 plates for the transporters recommended by the FDA and the EMA.
물리적 형태
Supplied as frozen membrane vesicles, containing 5 mg/ml membrane protein, labeled with volume, catalog number (transporter), date of production, protocols and necessary reagents sufficient for the analysis of 3 or 10, 96-well plates.
법적 정보
Distributed for SOLVO Biotechnology, Inc.
PREDEASY is a trademark of Solvo Biotechnology
PREDIVEZ is a trademark of Solvo Biotechnology
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
표적 기관
Respiratory system
Storage Class Code
3 - Flammable liquids
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
R Evers et al.
British journal of cancer, 83(3), 375-383 (2000-08-06)
The multidrug resistance proteins MRP1 and MRP2 are members of the same subfamily of ATP-binding cassette transporters. Besides organic molecules conjugated to negatively charged ligands, these proteins also transport cytotoxic drugs for which no negatively charged conjugates are known to
M G Donner et al.
Hepatology (Baltimore, Md.), 34(2), 351-359 (2001-08-02)
Cholestasis induces down-regulation of multidrug resistance protein 2 (Mrp2, symbol Abcc2), which is localized to the canalicular membrane. Given the overlapping substrate specificities of Mrp2 and multidrug resistance protein 3 (Mrp3, symbol Abcc3), we examined the hypothesis of a different
Thomas Rau et al.
Clinical pharmacology and therapeutics, 80(5), 468-476 (2006-11-23)
The adenosine triphosphate-binding cassette (ABC) class transporter ABCC2 (MRP2 [multidrug resistance related protein 2] or cMOAT [canalicular multispecific organic anion transporter]) is involved in the cellular outward transport and elimination of methotrexate. We hypothesized that common genetic variations may contribute
Isabelle Benz-de Bretagne et al.
Journal of biomedicine & biotechnology, 2011, 498757-498757 (2011-05-05)
MRP2 encoded by ABCC2 gene is involved in the secretion of numerous drugs and endogenous substrates. Patients with Dubin-Johnson syndrome due to mutation in ABCC2 gene have elevated urinary coproporphyrin ratio (UCP I/(I + III)). Here we investigated whether this
Potentiation of MRP2/Mrp2-mediated estradiol-17beta-glucuronide transport by drugs--a concise review.
Krisztina Herédi-Szabó et al.
Chemistry & biodiversity, 6(11), 1970-1974 (2009-11-26)
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