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Merck
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Key Documents

SAE0005

Sigma-Aldrich

Glucose-6-phosphate Isomerase human

recombinant, expressed in E. coli

동의어(들):

GPI, PGI

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About This Item

효소 위원회 번호:
UNSPSC 코드:
12352204
NACRES:
NA.54

재조합

expressed in E. coli

Quality Level

분석

≥95% (SDS-PAGE)

특이 활성도

≥200 units/mg protein

저장 온도

−20°C

생화학적/생리학적 작용

Glucose-6-phosphate isomerase (GPI) is a homodimeric enzyme ubiquitously present in most organisms. GPI catalyzes the interconversion between glucose-6-phosphate and fructose-6-hosphate, the second step of the glycolytic pathway. In mammals, Glucose-6-phosphate isomerase also acts as an autocrine motility factor (AMF), a neuroleukin, and a maturation factor. GPI deficiency is the second most common erythroenzymopathy of glycolytic enzymes after pyruvate kinase deficiency. Inherited deficiency of the enzymatic activity of GPI causes hereditary nonspherocytic hemolytic anemia (HNSHA) in humans, a severe deficiency that can be associated with hydrops fetalis (a-thalassaemia), immediate neonatal death, and neurological impairment.

물리적 특성

C-terminal histidine-tagged

단위 정의

One unit will convert 1.0 mmole of D-fructose 6-phosphate to D-glucose 6-phosphate per inute at pH 7.4 at 25 °C.

물리적 형태

Supplied as a solution in 50 mM Tris-HCl pH 7.5, and 50% glycerol

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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