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Merck
모든 사진(1)

주요 문서

SAB4200791

Sigma-Aldrich

Anti-Human IgG (Fab specific)-Peroxidase antibody, Mouse monoclonal

clone GG-6, purified from hybridoma cell culture

동의어(들):

Anti-Human immunoglobulin G

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.46

생물학적 소스

mouse

Quality Level

결합

peroxidase conjugate

항체 형태

purified from hybridoma cell culture

항체 생산 유형

primary antibodies

클론

GG-6, monoclonal

양식

lyophilized powder

종 반응성

human

농도

~2 mg/mL

기술

direct ELISA: 1:16,000-1:32,000 using 2.5 μg/ml human IgG for coating.

동형

IgG1

배송 상태

dry ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

일반 설명

Anti-Human IgG (Fab specific)-Peroxidase antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the GG-6 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from mouse immunized with purified human IgG (Fab fragment). IgGs are the most common Immunoglobulins isotype in blood, lymph fluid, cerebrospinal fluid and peritoneal fluid and a key players in the humoral immune response. IgGs include four subclasses (IgG1, IgG2, IgG3, and IgG4), they consist of a variable Fab fragment (which includes the antibody recognition site), and a conserved Fc fragment. The IgG subclasses differ in their physical and chemical properties, their distribution pattern is found to be age-dependent, and every subclass has a specific biological function.

면역원

Purified human IgG (Fab Fragment)

애플리케이션

Direct ELISA: a working dilution of 1:16,000-1:32,000 is recommended using 2.5 μg/mL human IgG for coating.

생화학적/생리학적 작용

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. Maternal IgG is the only antibody transported across the placenta to the fetus. Maternal IgG passively immunizes the infants. IgG deficiencies are often associated with various diseases.

물리적 형태

Supplied as a lyophilized powder.

기타 정보

In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.

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픽토그램

Exclamation mark

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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시험 성적서(COA)

Lot/Batch Number

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문서 라이브러리 방문

R Jefferis et al.
Annales de biologie clinique, 52(1), 57-65 (1994-01-01)
Secondary systemic immune responses are predominantly of the IgG class and passive administration of intravenous IgG, from pooled normal serum, is an effective prophylactic and/or therapeutic treatment for patients with defined immunodeficiencies. However, the proportions of each IgG subclass present
C B Reimer et al.
Hybridoma, 3(3), 263-275 (1984-01-01)
Stable clones of 31 mouse hybridomas that produce monoclonal antibodies (MAbs) against human IgG antigenic determinants were obtained. The number of hybridomas of different specificity described are: 2 anti-IgG1 Fc, 1 anti-IgG2 Fc, 1 anti-IgG2 Fd, 2 anti-IgG3 Fc, 2
C Papadea et al.
Critical reviews in clinical laboratory sciences, 27(1), 27-58 (1989-01-01)
Human IgG consists of two identical heavy (H) chains and two identical light (L) chains joined by interchain disulfide bridges. Heterogeneity in the amino acid sequences of the H and L polypeptides results in at least three types of IgG
Human placental Fc receptors and the transmission of antibodies from mother to fetus.
Simister NE and Story CM
Journal of Reproductive Immunology, 37(1), 1-23 (1997)
Specific IgG for cat allergens in patients with allergic conjunctivitis.
Miyama A, et al.
International Ophthalmology, 35(4), 575-586 (2015)

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