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Merck
모든 사진(4)

주요 문서

SAB4200732

Sigma-Aldrich

Anti-β-Tubulin I antibody, Mouse monoclonal

clone SAP.4G5, purified from hybridoma cell culture

동의어(들):

Anti-TUBB

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.43

생물학적 소스

mouse

항체 형태

purified from hybridoma cell culture

항체 생산 유형

primary antibodies

클론

SAP.4G5, monoclonal

양식

buffered aqueous solution

분자량

~55 kDa

종 반응성

bovine, gerbil, Xenopus, human, canine, mouse, hamster, chicken, rat, monkey

농도

~1.0 mg/mL

기술

immunoblotting: 0.06-0.12 μg/mL using HeLa cell extract
immunofluorescence: 1-2.5 μg/mL using HeLa cells
immunohistochemistry: 1-2 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human fallopian tube or testis sections.

동형

IgG1

UniProt 수납 번호

배송 상태

dry ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

유전자 정보

human ... TUBB(203068)

일반 설명

Monoclonal Anti-β-Tubulin I (mouse IgG1 isotype) is derived from the SAP.4G5 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse. Tubulin is the major building block of microtubules, an intracellular cylindrical filamentous structure, that is present in almost all eukaryotic cells. Tubulin exists in all cells as a 100 kDa protein, which assembles from heterodimers of α-tubulin and β-tubulin; both have a molecular weight of 55 kDa and share considerable homology.

면역원

synthetic peptide corresponding to the C-terminal sequence of ß-tubulin isotype I, conjugated to BSA

애플리케이션

Anti-β-Tubulin I antibody, Mouse monoclonal has been used in:
  • immunoblotting
  • immunofluorescence
  • immunohistochemistry

생화학적/생리학적 작용

β-Tubulin I is also useful in deducing the role that different tubulin isotypes play in nerve development, function or maintenance.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서 라이브러리 방문

Brian Perry et al.
Journal of the Association for Research in Otolaryngology : JARO, 4(3), 329-338 (2003-12-24)
The seven mammalian isotypes of beta tubulin are strikingly similar in amino acid sequence. The differences in isotypic sequence, although small, are nonetheless conserved in evolution, which suggests that they may confer distinct functional roles. If so, such roles should
Tubulin modifications and their cellular functions
Hammond JW, et al.
Current Opinion in Cell Biology, 20(1), 71-76 (2008)
S S Siddiqui et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 9(8), 2963-2972 (1989-08-01)
We have identified 2 anti-tubulin monoclonal antibodies that bind to 2 different subpopulations of identified neurons in the simple nervous system of the nematode Caenorhabditis elegans. The antibodies also recognize specific tubulin isotypes from C. elegans that were separated by
M C Roach et al.
Cell motility and the cytoskeleton, 39(4), 273-285 (1998-05-15)
Tubulin, the subunit protein of microtubules, is an alpha/beta heterodimer. In many organisms, both alpha and beta consist of various isotypes. Although the isotypes differ in their tissue distributions, the question of whether the isotypes perform different functions in vivo

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