T5326

Anti-γ-Tubulin antibody, Mouse monoclonal

clone GTU-88, purified from hybridoma cell culture

• 동의어(들): Anti-Tubulin Antibody
• MDL number: MFCD00677366
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 항체 형태: purified antibody (purified from hybridoma cell culture)
• 항체 생산 유형: primary antibodies
• 클론: GTU-88, monoclonal
• 형태: buffered aqueous solution
• 분자량: antigen 48 kDa
• 종 반응성: rat, hamster, chicken, human, bovine, canine, Xenopus, mouse
• 포장: antibody small pack of 25 μL
• 향상된 검증: independent; Learn more about Antibody Enhanced Validation
• 농도: ~1 mg/mL
• 기술: immunocytochemistry: 1-2 μg/mL using HeLa cells; immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 1-2 μg/mL using total cell extract of chicken fibroblasts
• 동형: IgG1
• UniProt 수납 번호: P23258
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... TUBG1(7283) ; mouse ... Tubg1(103733) ; rat ... Tubg1(252921)

설명

일반 설명

γ-Tubulin, mapped on human chromosome 17q21.2, codes for a member of the tubulin family. It is localized to the microtubule organizing centres. γ-Tubulin consists of two isoforms tubulin γ 1 (TUBG1) and tubulin γ 2 (TUBG2) with 97.3% amino acid identity. In addition to these two isoforms, γ-tubulin pseudogene (TUBG1P) is also been identified on human chromosome 17. Human TUBG1 and TUBG2 transcripts are widely expressed in preimplantation embryos and the brain, respectively. γ-tubulin is a component of γ-tubulin ring complex (γ-TuRC), which has roughly the same diameter as a microtubule.

특이성

The antibody recognizes an epitope located within the N-terminal region of γ-tubulin.

면역원

synthetic γ-tubulin peptide, conjugated to KLH

애플리케이션

Monoclonal Anti-γ-Tubulin antibody produced in mouse has been used in following studies:

• Western blot analysis
• Immunohistochemistry
• Immunocytology

생화학적/생리학적 작용

γ-Tubulin nucleates microtubule assembly throughout the mammalian cell cycle in vivo. In Aspergillus nidulans, γ-tubulin facilitates attachment of microtubules to the spindle pole body, nuclear division and microtubule assembly. Ubiquitination of γ-tubulin by breast cancer 1 protein (BRCA1) is a crucial step in the regulation of centrosome number. Overexpression of γ-tubulin is observed in lung cancer. Tubulin γ 2 (TUBG2) plays a vital role in cell growth. Aberration in the γ-tubulin gene alters microtubule assembly. The expression levels of γ-tubulin can be considered as an important prognostic indicator for patients with astrocytomas.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

저장 및 안정성

Store at –20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)