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Merck
모든 사진(1)

주요 문서

SAB4200702

Sigma-Aldrich

Anti-Chicken Egg Albumin (Ovalbumin) antibody, Mouse monoclonal

clone OVA-14, purified from hybridoma cell culture

동의어(들):

Albumin, Plakalbumin, chicken egg

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.46

생물학적 소스

mouse

Quality Level

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

OVA-14, monoclonal

양식

buffered aqueous solution

분자량

antigen 45 kDa

종 반응성

chicken, turkey

농도

~1 mg/mL

기술

competitive inhibition ELISA: suitable
dot blot: suitable
immunoblotting: suitable
indirect ELISA: 0.1-0.2 μg/mL using 10 μg/mL Albumin from chicken egg white (A5503) for coating.

동형

IgG1

배송 상태

dry ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

유전자 정보

chicken ... Oval(396058)

일반 설명

Anti-Chicken egg albumin (ovalbumin) antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the hybridoma OVA-14 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified chicken egg albumin. The product reacts with turkey egg albumin, but not with serum albumin of the following species: chicken, turkey, human, bovine, pig, donkey, goat, sheep, horse, dog, cat, guinea pig, rabbit, rat, mouse, and pigeon.
Ovalbumin (323-339) is the most abundant egg white protein surrounded by B-cell epitopes, which are bound by specific IgE antibodies. In addition, it also contains CD4+ T cell epitopes. Ovalbumin is a monomeric protein with a molecular mass of 45000Da.

면역원

purified Chicken Egg Albumin

생화학적/생리학적 작용

Ovalbumin (OVA) peptide 323-339 mediates 25–35% of T-cell response to intact OVA in isolated BALB/c mouse. OVA 323-339 has been widely used to determine the characteristics of class II major histocompatibility complex (MHC)-peptide binding and T-cell activation.

물리적 형태

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

저장 및 안정성

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

기타 정보

In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서 라이브러리 방문

D E Briles et al.
Infection and immunity, 68(2), 796-800 (2000-01-20)
Acquisition of pneumococci is generally from carriers rather than from infected individuals. Therefore, to induce herd immunity against Streptococcus pneumoniae it will be necessary to elicit protection against carriage. Capsular polysaccharide-protein conjugates, PspA, and PsaA are known to elicit some
Pathum Dhanapala et al.
Molecular immunology, 66(2), 375-383 (2015-05-13)
Hypersensitivity to the chicken egg is a widespread disorder mainly affecting 1-2% of children worldwide. It is the second most common food allergy in children, next to cow's milk allergy. Egg allergy is mainly caused by hypersensitivity to four allergens
The Complete Amino-Acid Sequence of Hen Ovalbumin
Nisbet AD, et al.
European Journal of Biochemistry, 115, 335-345 (1981)
Caubet JC. and Wang J .,
Pediatric Clinics of North America, 58, 427-43 (2011)
Caroline Montelius et al.
The British journal of nutrition, 106(6), 836-844 (2011-07-09)
Thylakoid membranes, derived from chloroplasts, have previously been shown to retard fat digestion and lower blood glucose levels after oral intake. The purpose of the present study was to investigate the effect of thylakoid membranes on the passage of methyl-glucose

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