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Merck
모든 사진(3)

Key Documents

SAB4200661

Sigma-Aldrich

Monoclonal Anti-Dynamin 2 (DNM2) antibody produced in mouse

clone DYN2-11, purified from hybridoma cell culture

동의어(들):

CMT2M, CMTDI1, CMTDIB, DI-CMTB, DYN2, DYNII, LCCS5, dynamin 2

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

DYN2-11

형태

buffered aqueous solution

분자량

antigen ~98 kDa

종 반응성

mouse, human, monkey, canine, rat

농도

~1 mg/mL

기술

immunoblotting: 2-4 μg/mL using using whole extract of HeLa cells.
immunofluorescence: 5-10 μg/mL using using HeLa cells.
immunoprecipitation (IP): 5-10 μg using using whole extract of HeLa cells.

동형

IgG1

UniProt 수납 번호

저장 온도

−20°C

타겟 번역 후 변형

unmodified

유전자 정보

human ... DNM2(1785)
mouse ... Dnm2(13430)
rat ... Dnm2(25751)

일반 설명

Monoclonal Anti-Dynamin 2 (DNM2) (mouse IgG1 isotype) is derived from the hybridoma DYN2-11 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Dynamin 2 (DNM2) also known as DYN2 belongs to the dynamins family of guanosine triphosphatase (GTPase) proteins.
The gene DNM2 (dynamin 2) is mapped to human chromosome 19p13. It is widely expressed. The protein has a catalytic amino-terminal GTPase domain, a middle domain, a pleckstrin homology domain (PH), a GTPase effector domain (GED) and a less conserved carboxyl-terminal proline/arginine rich domain (PRD).

면역원

synthetic peptide corresponding to a sequence at the N-terminal region of human DNM2 , conjugated to KLH

애플리케이션

Monoclonal Anti-Dynamin 2 (DNM2) antibody produced in mouse has been used in:
  • immunoblotting
  • immunoprecipitation
  • immunofluorescence

생화학적/생리학적 작용

DNM2 (dynamin 2) is a GTPase which is required for clathrin-mediated endocytosis. It is important for clathrin-coated pit maturation and clathrin-coated vesicle formation. DNM2 participates in neuronal morphology, axonal growth, centrosome cohesion, actin- and microtubular organization. Mutations in it are associated with Charcot-Marie-Tooth disease, centronuclear myopathy ADCNM (autosomal dominant centronuclear myopathy) and lethal congenital contractures syndrome type 5 (LCCS5). It is upregulated in prostate cancer.
Furthermore, DNM2 is upregulated in pancreatic cancer as it activates Ras-related C3 botulinum toxin substrate 1 (Rac1). It also promotes invasive cellular migration and lamellipod protrusion.

물리적 형태

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

Dynamins at a glance.
Jürgen A W Heymann et al.
Journal of cell science, 122(Pt 19), 3427-3431 (2009-09-18)
Arlek M González-Jamett et al.
Frontiers in endocrinology, 4, 126-126 (2013-09-26)
Dynamin-2 is a ubiquitously expressed mechano-GTPase involved in different stages of the secretory pathway. Its most well-known function relates to the scission of nascent vesicles from the plasma membrane during endocytosis; however, it also participates in the formation of new
Multiple Dynamin family members collaborate to drive mitochondrial division
Lee JE, et al.
Nature, 540(7631), 139-139 (2016)
Shawn M Ferguson et al.
Nature reviews. Molecular cell biology, 13(2), 75-88 (2012-01-12)
Dynamin, the founding member of a family of dynamin-like proteins (DLPs) implicated in membrane remodelling, has a critical role in endocytic membrane fission events. The use of complementary approaches, including live-cell imaging, cell-free studies, X-ray crystallography and genetic studies in
Bin Xu et al.
Cancer medicine, 3(1), 14-24 (2014-01-10)
Dynamin 2 (Dyn2) is essential for intracellular vesicle formation and trafficking, cytokinesis, and receptor endocytosis. In this study, we investigated the implication of Dyn2 as a prognostic marker and therapeutic target for progressive prostate cancer (PCA). We evaluated Dyn2 protein

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