SAB4200500

Anti-Collagen IV antibody, Mouse monoclonal

clone J3-2, purified from hybridoma cell culture

• 동의어(들): Anti-COL4, Monoclonal Anti-Collagen IV antibody produced in mouse
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified from hybridoma cell culture
• 항체 생산 유형: primary antibodies
• 클론: J3-2, monoclonal
• 형태: buffered aqueous solution
• 종 반응성: mouse, rat, dog, bovine, human, monkey
• 농도: ~1.0 mg/mL
• 기술: immunohistochemistry: 2.0-4.0 μg/mL using human liver tissue sections.; western blot: 1.0-2.0 μg/mL using human placenta extracts.
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... COL4A1(1282)

설명

일반 설명

Human collagen IV comprises six genes. The COL4A1-COL4A2 is mapped to chromosome 13. The COL4A3-COL4A4 and COL4A5-COL4A6 are localized on chromosome 2 and chromosome X, respectively. It belongs to the collagen superfamily and is associated with the basement membranes (BMs). The six α-chains namely α1(IV) to α6(IV) comprises an N-terminal cysteine and lysine domain, a typical Gly-X-Y triple repeats and a C-terminal noncollagenous (NC1) domain.

면역원

placenta preparation rich in basement membrane collagen.

애플리케이션

Monoclonal Anti-Collagen IV antibody produced in mouse has been used:

• in the immunohistochemical staining
• in the immunostaining
• in the immunofluorescence detection
• as a secondary antibody in the western blot analysis

생화학적/생리학적 작용

Collagen IV plays a key role in basement membrane assembly. The six chains assemble as three different protomers namely, the α 1.α1.α2(IV), α3.α4.α5(IV) and α 5.α5.α6(IV). The trimers are formed in different combinations of the α-chains. High expression levels of α3, α4 and α5 is observed in specialized glomerular BM (GBM). Mutations in the COlA5 gene is associated with Alport syndrome and α3(IV) chain is associated pathogenesis of Goodpasture syndrome, an autoimmune disease. Mutation in the COlA5 is also implicated in Diffuse Esophageal Lewmyomatosis.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable