콘텐츠로 건너뛰기
Merck
모든 사진(3)

Key Documents

SAB4200372

Sigma-Aldrich

Anti-WASH1 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

동의어(들):

Anti-FAM39E, Anti-WAS protein family homolog 1, Anti-WASH

로그인조직 및 계약 가격 보기


About This Item

UNSPSC 코드:
12352203
NACRES:
NA.41

생물학적 소스

rabbit

결합

unconjugated

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

클론

polyclonal

형태

buffered aqueous solution

분자량

antigen ~72 kDa

종 반응성

human, rat, mouse

농도

~1.0 mg/mL

기술

immunoprecipitation (IP): 5-10 μg using lysates of rat NRK cells.
western blot: 2-4 μg/mL using whole extracts of HEK-293T cells over-expressing mouse WASH1.

배송 상태

dry ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

유전자 정보

일반 설명

WASH1 (Wiskott-Aldrich Syndrome Protein and SCAR Homolog) is a new member of the WASP family. Similar to other WASP family members, it contains a C-terminal for ′WH2, connecting and acidic′ (WCA) domain that binds to Actin related protein 2/actin related protein 3 (Arp2/3). In addition, WASH1 also contains a short proline-rich region, a unique N-terminal domain termed WASH-homology domain (WAHD1), and a tubulin-binding region.

면역원

synthetic peptide corresponding to an internal region of human WASH1, conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat.

애플리케이션

Anti-WASH1 antibody produced in rabbit has been used in western blotting and immunoprecipitation.

생화학적/생리학적 작용

WASH1 (Wiskott-Aldrich Syndrome Protein and SCAR Homolog), is a nucleation-promoting factor at the surface of endosomes. It recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of endosomes. WASH1 forms part of a multiprotein complex composed of FAM21, KIAA1033 strumpellin and WASH-interacting protein (SWIP) and coiled coil domain containing 53 (CCDC53). It associates with tubulin and localizes to early and recycling endosomes, where together with the Arp2/3 complex and actin, it is required for maintaining the shape of the endosomal compartment and the regulation of the retrograde transport.

물리적 형태

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

적합한 제품을 찾을 수 없으신가요?  

당사의 제품 선택기 도구.을(를) 시도해 보세요.

Storage Class Code

10 - Combustible liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Missense variant in CCDC22 causes X-linked recessive intellectual disability with features of Ritscher-Schinzel/3C syndrome
Kolanczyk M, et al.
European Journal of Human Genetics, 23(5), 633-633 (2015)
DNAJC13 p. Asn855Ser, implicated in familial parkinsonism, alters membrane dynamics of sorting nexin 1
Follett J, et al.
Neuroscience Letters, 706(5), 114-122 (2019)
Mateusz Kolanczyk et al.
European journal of human genetics : EJHG, 23(5), 633-638 (2014-06-12)
Ritscher-Schinzel syndrome (RSS)/3C (cranio-cerebro-cardiac) syndrome (OMIM#220210) is a rare and clinically heterogeneous developmental disorder characterized by intellectual disability, cerebellar brain malformations, congenital heart defects, and craniofacial abnormalities. A recent study of a Canadian cohort identified homozygous sequence variants in the
WASH and the Arp2/3 complex regulate endosome shape and trafficking
Duleh SN and Welch MaD
Cytoskeleton (Hoboken, N.J.), 67(3), 193-206 (2010)
A FAM21-containing WASH complex regulates retromer-dependent sorting
Gomez TS and Billadeau DD
Developmental Cell, 17(5), 699-711 (2009)

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.