추천 제품
생물학적 소스
rabbit
결합
unconjugated
항체 형태
affinity isolated antibody
항체 생산 유형
primary antibodies
클론
polyclonal
형태
buffered aqueous solution
분자량
antigen ~218 kDa
농도
~1.0 mg/mL
기술
immunoprecipitation (IP): 2.5-5 μg using HeLa cell lysates
indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde fixed HeLa cells
western blot: 3-6 μg/mL using HeLa cell lysates
배송 상태
dry ice
저장 온도
−20°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... DICER1(23405)
mouse ... Dicer1(192119)
일반 설명
Dicer, also known as Dicer1, endoribonuclease Dicer, Helicase with RNase motif is a member of the RNase III family. It contains a N-terminal DEXH-box RNA helicase/ ATPase domain, followed by a domain of unknown function (DUF283), a Piwi/Argonaut/Zwill (PAZ ) domain, which anchors the 3′-end of RNA substrates.
Dicer1 is encoded by the gene mapped to human chromosome 14q32.13.
특이성
Anti-Dicer recognizes human Dicer.
애플리케이션
Anti-Dicer antibody produced in rabbit has been used in:
- immunoblotting
- immunoprecipitation
- immunofluorescent staining
생화학적/생리학적 작용
Dicer catalyzes the first step in the RNA interference (RNAi) pathway and initiates formation of the RNA-induced silencing complex (RISC). Dicer processes the dsRNA into small fragments called short interfering RNA (siRNA) or microRNA (miRNA) of typically 21-25 nucleotides long with a two-base overhang on the 3′. It helps to load these fragments into the RNA-induced silencing complex (RISC), which guides the sequence-specific silencing of mRNAs that contain complementary sequences by either enzymatically cleaving the mRNA or repressing its translation.
Mutation in the Dicer gene causes malignancies.
물리적 형태
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
저장 및 안정성
Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
The dynamic recruitment of TRBP to neuronal membranes mediates dendritogenesis during development
EMBO Reports, 19(3) (2018)
Dicer cleaves 5?-extended microRNA precursors originating from RNA polymerase II transcription start sites
Nucleic Acids Research, 46(11), 5737-5752 (2018)
Molecular mechanisms of Dicer: endonuclease and enzymatic activity
The Biochemical Journal, 474(10), 1603-1618 (2017)
Evolutionarily conserved roles of the dicer helicase domain in regulating RNA interference processing
The Journal of Biological Chemistry, 289(41), 28352-28362 (2014)
RNAi pathway participates in chromosome segregation in mammalian cells
Cell discovery, 1, 15029-15029 (2015)
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