추천 제품
Grade
for molecular biology
Quality Level
분석
>95% (SDS-PAGE)
형태
liquid
색상
colorless
외래 활성
DNase and RNase, none detected
배송 상태
wet ice
저장 온도
−20°C
일반 설명
Single-strand Binding Protein is a homotetramer, found in E. coli during the event of DNA metabolism.
애플리케이션
Binds with high specificity to single-stranded DNA. Can be useful in enhancing the specificity of PCR and in enabling the sequencing of problematic DNA templates.
Single-strand Binding Protein from Escherichia coli has been used in in vitro RNA/Single-Strand Binding Protein (SSB) complex formation assays and in protein purification to determine integrity and functionality of the integron attC sites.
생화학적/생리학적 작용
Single-strand Binding Protein (SSBP) exhibits high affinity towards single stranded DNA important for DNA metabolic processes. Single-stranded DNA-binding protein hSSB1 is critical for genomic stability and assists the delivery of primers during the formation of lagging standard. It also prevents the formation of inhibitory secondary structures in the exposed lagging-strand of DNA during replication. SSBP mediates the recruitment of other proteins to their sites of function on DNA.
물리적 형태
Solution in 20 mM Tris-HCl, pH 8.0, 0.5 M NaCl, 0.1 mM EDTA, 0.1 mM DTT, 50% glycerol
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Structure of the SSB--DNA polymerase III interface and its role in DNA replication
Marceau AH, et al.
The Embo Journal, 30(20), 4236-4247 (2011)
Binding Specificity of E. coli SSB protein for the ? subunit of DNA pol III Holoenzyme and PriA helicase
Alexander GK, et al.
Biochemistry, 49(17), 3555-3556 (2010)
J Kieleczawa et al.
Science (New York, N.Y.), 258(5089), 1787-1791 (1992-12-11)
When template DNA is saturated with a single-stranded DNA binding protein (SSB), strings of three or four contiguous hexanucleotides (hexamers) can cooperate through base-stacking interactions to prime DNA synthesis specifically from the 3' end of the string. Under the same
The Integron Integrase Efficiently Prevents the Melting Effect of Escherichia coli Single-Stranded DNA-Binding Protein on Folded attC Sites
Loot C, et al.
Journal of Bacteriology, 196(4), 762-771 (2014)
Minimizing deletion mutagenesis artifact during Taq DNA polymerase PCR by E. coli SSB.
Q Chou
Nucleic acids research, 20(16), 4371-4371 (1992-08-25)
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