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RECOMT

Sigma-Aldrich

Thrombin CleanCleave™ Kit

동의어(들):

Thrombin Cleavage Kit

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About This Item

UNSPSC 코드:
12352200
NACRES:
NA.56

형태

suspension

Quality Level

200

분자량

37 kDa

기술

protein purification: suitable

배송 상태

wet ice

저장 온도

2-8°C

일반 설명

The Thrombin CleanCleave Kit contains a 50% suspension fo thrombin-agarose produced by immobilizing bovine thrombin and is designed for cleavage of recombinant fusion proteins

애플리케이션

For cleaving a tag from a recombinant fusion protein which contains the thrombin recognition sequence. Thrombin-agarose is compatible with recombinant proteins expressed in various types of expression systems.

특징 및 장점

  • Fast, efficient cleavage in as little as 2 hours
  • Thrombin is covalently bound to agarose for easy removal.
  • The robust cleavage reaction is effective at temperatures from 4 °C to 37 °C and over a wide range of pH and ionic strengths.
  • Cleave tags even in the presence of 0.1% Triton™, 1 M urea or 5 mM EDTA
  • Thrombin-agarose is reusable.

수량

200 μl of a 50% slurry of thrombin-agarose cleaves >85% of 1 mg of fusion protein.

법적 정보

CleanCleave is a trademark of Sigma-Aldrich Co. LLC
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

키트 구성품 전용

제품 번호
설명
  • Thrombin Cleavage Buffer, 10× 10 mL

관련 제품

제품 번호
설명
가격

08168

Timestrip Plus™ 8 °C

Storage Class Code

10 - Combustible liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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