추천 제품
환경친화적 대안 제품 특성
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
환경친화적 대안 카테고리
, Aligned
배송 상태
ambient
저장 온도
room temp
일반 설명
Sigma′s GenElute™ RNA/DNA/Protein Purification Plus Kit provides a rapid method for the isolation and purification of total RNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants. The total RNA, genomic DNA and proteins are all column purified in less than 30 minutes. This kit is ideal for researchers who are interested in studying the genome, proteome and transcriptome of a single sample, such as for studies of microRNA profiling, gene expression including gene silencing experiments or mRNA knockdowns, studies involving biomarker discovery, and for characterization of cultured cell lines. Sigma′s GenElute™ RNA/DNA/Protein Purification Plus Kit is especially useful for researchers who are isolating macromolecules from precious, difficult to obtain or small samples such as biopsy materials or single foci from cell cultures, as it eliminates the need to fractionate the sample. Furthermore, analysis will be more reliable since the RNA, DNA and proteins are derived from the same sample, thereby eliminating inconsistent results. The purified macromolecules are of the highest purity and can be used in a number of different downstream applications
- Sequentially isolate and purify total RNA, genomic DNA and total proteins from a single sample
- Ideal for small or difficult to obtain samples
- No need to split the lysate, or to use phenol or precipitation methods
- Purify RNA/DNA/Protein from cultured animal cells, tissues, blood, bacteria, yeast, fungi or plants
- Rapid and efficient spin column procedure
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
애플리케이션
RNA
Genomic DNA
Protein
- Bioanalyzer
- Quantitative, real-time RT-PCR for both large mRNA and small RNA including miRNA
- RT-PCR for both large mRNA and small RNA including miRNA
- Northern blotting
- RNase protection
- Primer extension
- Expression array assays
- Next Generation Sequencing
- microRNA Cloning
- PCR-based virus detection
- PCR-based viable bacteria detection
- miRNA array
- RNA-Seq
- Nanostring
- Fluidigm
- Droplet/Digital PCR
Genomic DNA
- PCR and Quantitative PCR
- Sequencing
- DNA Methylation Studies
- Southern blotting
- Microarrays
- SNP analysis
- PCR-based virus detection/identification
- PCR-based bacteria detection/identification
Protein
- SDS-PAGE analysis
- Western blots
- Mass Spectrometry
특징 및 장점
- Complete column purification - The RNA, DNA and proteins are all column purified
- Reduce variability - RNA, DNA and proteins are isolated from a single sample with no splitting of the lysate, thus reducing inconsistent results and variability
- Isolate from small samples - Sequential isolation of RNA, DNA and protein from a single sample. Ideal for precious, difficult to obtain or small samples such as biopsy material or single foci from cell cultures.
- Rapid procedure - Isolate total RNA, genomic DNA and total proteins from a single sample in less than 30 minutes
- Isolate a diversity of RNA species - All sizes of RNA are isolated, from large mRNA down to microRNA
- Isolate high quality RNA, DNA and proteins - The purified RNA, DNA and proteins are of the highest quality and can be used in a number of downstream applications
단위 정의
Preps
법적 정보
GenElute is a trademark of Sigma-Aldrich Co. LLC
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Acute Tox. 3 Inhalation - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A - STOT SE 3
표적 기관
Respiratory system
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Chenyang Jiang et al.
Nature, 585(7824), 261-267 (2020-08-28)
Sustained, drug-free control of HIV-1 replication is naturally achieved in less than 0.5% of infected individuals (here termed 'elite controllers'), despite the presence of a replication-competent viral reservoir1. Inducing such an ability to spontaneously maintain undetectable plasma viraemia is a
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.