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Merck
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PROTTWO

Sigma-Aldrich

ProteoPrep® Universal Extraction Kit

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About This Item

UNSPSC 코드:
12352200
NACRES:
NA.32

형태

dry powder

Quality Level

200

저장 온도

2-8°C

관련 카테고리

애플리케이션

This kit features new and innovative detergents, and uses specially formulated reagents and an optimized protocol designed to generate two prepared subcellular fractions that are uniquely ready for two-dimensional (2D) electrophoresis.
  • Fraction 1: Soluble/Cytoplasmic Proteins
  • Fraction 2: Membrane Proteins
The special reducing and alkylating reagents produce samples that exhibit improved focusing and decreased streaking in 2D gels. The ProteoPrep Universal Extraction Kit was designed through a collaboration of Proteome Systems and Sigma research scientists.

특징 및 장점

  • Innovative detergent preparations - Highly improved solubility allows for higher protein loads and greater visibility of low abundance proteins on 2D gels.
  • Two pre-mixed solubilizaton solutions - Generates two distinct populations for easy 2D analysis.
  • Pre-measured reducing and alkylating reagents - Easy-to-use reagents provide improved IEF resolution.
  • Pre-weighed dry blends - Stable and easy to reconstitute
  • Conveniently Packaged - No waste. Use only the amount needed.

법적 정보

ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany

키트 구성품 역시 별도로 이용 가능함

제품 번호
설명
SDS
  • C0356Protein Extraction Reagent Type 4 23 mLSDS
  • T7567Tributylphosphine solution, 200 mM (in N-methyl-2-pyrrolidinone), liquid 5 x 0.5SDS
  • A3221Iodoacetamide, Single use vial of 56 mg 5 x 56SDS

신호어

Danger

Hazard Classifications

Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3

표적 기관

Respiratory system

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

W M Freeman et al.
Neuroscience, 159(1), 183-195 (2009-01-13)
Declining cognitive performance is associated with increasing age, even in the absence of overt pathological processes. We and others have reported that declining cognitive performance is associated with age-related changes in brain glucose utilization, long-term potentiation and paired-pulse facilitation, protein
Maria De Angelis et al.
Electrophoresis, 31(13), 2126-2136 (2010-07-02)
Food allergy is recognized as one of the major health concerns. It is estimated that ca. 4% of the population is affected by food allergenic disorders. Food allergies are defined as IgE-mediated hypersensitivity reactions. Foods such as peanuts, tree nuts
Diliana Dancheva Simeonova et al.
Molecular & cellular proteomics : MCP, 8(1), 122-131 (2008-08-22)
We present here a new approach that enabled the identification of a new protein from a bacterial strain with unknown genomic background using a combination of inverted PCR with degenerate primers derived from N-terminal protein sequences and high resolution peptide
Susanne K Pedersen et al.
Journal of proteome research, 2(3), 303-311 (2003-06-20)
Abundant and hydrophilic nonmembrane proteins with isoelectric points below pH 8 are the predominant proteins identified in most proteomics projects. In yeast, however, low-abundance proteins make up 80% of the predicted proteome, approximately 50% have pl's above pH 8 and
FcR? Presence in TCR Complex of Double-Negative T Cells Is Critical for Their Regulatory Function.
Thomson, C.W., et al.
Journal of Immunological Methods, 177, 2250-2257 (2006)
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