P8996

Monoclonal Anti-PINCH-1 antibody produced in mouse

clone PINCH-C58, purified immunoglobulin, buffered aqueous solution

• MDL number: MFCD06798713
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• 결합: unconjugated
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: PINCH-C58, monoclonal
• 양식: buffered aqueous solution
• 분자량: antigen ~37 kDa
• 종 반응성: hamster, bovine, mouse, monkey, canine, human, rat
• 기술: immunoprecipitation (IP): suitable; indirect ELISA: suitable; western blot: 1-2 μg/mL using total cell extract from 293T cells
• 동형: IgG1
• UniProt 수납 번호: P48059
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... LIMS1(3987) ; mouse ... Lims1(110829) ; rat ... Gcc2(309798)

설명

일반 설명

Monoclonal Anti-PINCH-1 (mouse IgG1 isotype) is derived from the hybridoma PINCH-C58 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a peptide of human PINCH1, conjugated to KLH. LIM and senescent cell antigen-like-containing domain protein 1 (LIMS1) or particularly interesting new Cys-His protein 1 (PINCH-1) is widely expressed. It has five LIM domains. It has leucine-rich nuclear export signal (NES) and nuclear localization signal (NLS) at the C-terminus. Human PINCH-1 and 2 are encoded by two different genes that are 82% identical in their amino acid sequence.

면역원

peptide corresponding to amino acids 281-299 of human PINCH-1, conjugated to KLH.

애플리케이션

Monoclonal Anti-PINCH-1 antibody produced in mouse has been used in:

• enzyme linked immunosorbent assay (ELISA)
• immunoblotting
• immunoprecipitation
• co-immunoprecipitation

생화학적/생리학적 작용

The interaction with PINCH proteins (PINCH-1 and 2) is important for localization of integrin linked kinase protein to cell matrix contact sites. It plays a key role in cell−matrix adhesion. PINCH2 is co-expressed and co-localized with PINCH-1/ILK complex. PINCH1 interacts with Wilms tumor 1 protein (WT1) in podocytes. Elevated expression of PINCH1 is observed in human laryngeal carcinoma.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable