P7965

Monoclonal Anti-P-Glycoprotein (MDR) antibody produced in mouse

clone F4, ascites fluid

• 동의어(들): Anti-ABC20, Anti-CD243, Anti-CLCS, Anti-GP170, Anti-MDR1, Anti-P-GP, Anti-PGY1, Anti-p-170
• MDL number: MFCD00282355
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: ascites fluid
• 항체 생산 유형: primary antibodies
• 클론: F4, monoclonal
• 분자량: antigen 170-180 kDa
• 포함: 15 mM sodium azide
• 종 반응성: hamster, human
• 기술: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human kidney sections; immunohistochemistry (frozen sections): suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; radioimmunoassay: suitable using cell-surface RIA; western blot: suitable
• 동형: IgG1
• UniProt 수납 번호: P08183
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... ABCB1(5243)

설명

일반 설명

The ABCB1 (ATP binding cassette subfamily B member 1) gene is mapped to human chromosome 7q21.12. It encodes for P-glycoprotein, which is a membrane bound drug transporter protein, belonging to the ATP-binding cassette (ABC) transporters family. The gene is considered to be highly polymorphic.

특이성

The antibody recognizes an epitope located in the amino terminal half of P-glycoprotein (Pgp), at the third extracellular loop of the molecule. The epitope is resistant to formalin fixation and periodate oxidation. The antibody detects specifically human MDR1 P-glycoprotein, but does not appear to recognize the human MDR3 product, nor the mouse mdr1a, mdr1b or the mdr3 P-glycoprotein.

면역원

mixture of human and hamster drug-resistant whole cells and crude plasma membranes.

애플리케이션

Monoclonal Anti-P-Glycoprotein (MDR) antibody produced in mouse has been used in western blot analysis and Immunohistochemistry.

생화학적/생리학적 작용

Overexpression of ABCB1 (ATP binding cassette subfamily B member 1) gene is a major cause for multi-drug resistance, which makes chemotherapy challenging for the treatment of osteosarcoma. P-glycoprotein (P-gp) mediates the energy-dependent efflux of xenobiotic to the outside of plasma membrane from the inside of the cell , thereby affecting the process of absorption, distribution, and excretion of drugs. Cyclosporine, a calcineurin inhibitor serves as a substrate for P-gp.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable