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Merck
모든 사진(1)

Key Documents

P7899

Sigma-Aldrich

Anti-Peroxidase antibody produced in rabbit

fractionated antiserum, lyophilized powder

동의어(들):

Rabbit Anti HRP

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.46

생물학적 소스

rabbit

Quality Level

결합

unconjugated

항체 형태

fractionated antiserum

항체 생산 유형

primary antibodies

클론

polyclonal

형태

lyophilized powder

종 반응성

horseradish

기술

indirect ELISA: 1:100,000-1:200,000

저장 온도

2-8°C

타겟 번역 후 변형

unmodified

일반 설명

Antiserum is determined to be immunospecific for horseradish peroxidase and does not inhibit the enzymatic activity of the peroxidase.
Peroxidase is a hemoprotein.
The fractionation procedure yields primarily the immunoglobulin fraction of antiserum. If necessary, the fractionated antiserum is adsorbed using solid phase techniques. Antiserum is determined to be immunospecific by immunoelectrophoresis (IEP), against purified and crude horseradish peroxidase
The gel is stained with DAB (diaminobenzidine) to ensure that the antibody does not inhibit the enzymatic activity of the peroxidase. Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the antibody preparation followed by diffusion against anti-rabbit IgG and anti-rabbit whole serum results in single arcs of precipitation in the γ region
Each mL will precipitate a minimum of 0.25 mg of peroxidase at equivalence. May be used to stain axons and synaptic terminals in Drosophila embryo fillets.

면역원

Purified peroxidase from horseradish

애플리케이션

Anti-Peroxidase antibody produced in rabbit has been used in:
  • immunoblot assays
  • enzyme-linked-immunosorbent assay (ELISA)
  • immunoprecipitation

생화학적/생리학적 작용

Peroxidase helps in catalyzing the oxidation of a number of substrates with the help of hydrogen peroxide.

물리적 형태

Lyophilized from 0.01 M phosphate buffered saline, pH 7.2

재구성

Reconstitute with deionized water.

분석 메모

Specificity is determined by immunoelectrophoresis using a crude peroxidase extract.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

Rudi Loesel et al.
Journal of neuroscience methods, 155(2), 202-206 (2006-02-17)
Being able to discriminate between neurons and non-neuronal cells such as glia and tracheal cells has been a major problem in insect neuroscience, because glia-specific antisera are available for only a small number of species such as Drosophila melanogaster and
Tyrosine nitration of cytosolic peroxidase is probably triggered as a long distance signaling response in sunflower seedling cotyledons subjected to salt stress
Jain P and Bhatla SC
PLoS ONE, 13(5), e0197132-e0197132 (2018)
Bin Yang et al.
Oncology letters, 15(4), 4662-4668 (2018-03-16)
The present study aimed to assess whether different anesthesia methods (general anesthesia and general anesthesia combined with epidural block) were associated with tumor metastasis during the perioperative period and the possible molecular mechanisms of tumor metastasis. A rat hepatoma tumor
Chilling injury and peroxidase activity changes in ?Fortune? mandarin fruit during low temperature storage
El-HF, et al.
Bulgarian Journal of Plant Physiology, 29(1-2), 44-54 (2003)
Mary Lynn Dear et al.
Development (Cambridge, England), 143(1), 75-87 (2015-11-26)
Synaptogenesis requires orchestrated intercellular communication between synaptic partners, with trans-synaptic signals necessarily traversing the extracellular synaptomatrix separating presynaptic and postsynaptic cells. Extracellular matrix metalloproteinases (Mmps) regulated by secreted tissue inhibitors of metalloproteinases (Timps), cleave secreted and membrane-associated targets to sculpt

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