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Merck
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문서

P7181

Sigma-Aldrich

Polyethylene glycol solution

Hybri-Max, 50 % (w/v), average mol wt 1,450, 0.2 μm filtered, BioReagent, suitable for hybridoma

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About This Item

MDL number:
UNSPSC 코드:
12161902
PubChem Substance ID:

Grade

Hybri-Max

Quality Level

무균

0.2 μm filtered

제품 라인

BioReagent

형태

solution

분자량

average mol wt 1,450

농도

50 % (w/v)

기술

cell culture | hybridoma: suitable

불순물

endotoxin, tested

저장 온도

2-8°C

SMILES string

C(CO)O

InChI

1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2

InChI key

LYCAIKOWRPUZTN-UHFFFAOYSA-N

일반 설명

Polyethylene glycol (PEG) is a polymer of ethylene oxide and water, obtained upon condensation.

애플리케이션

Polyethylene glycol solution has been used in the hybridization of the following:
  • irradiated camel fibroblast cells with A23 hamster cells
  • mouse splenocytes with Sp2/0-Ag 14 mouse myeloma cells
  • rabbit splenocytes and cell line 240E-1

Recommended for use in a normal fusion protocol requiring 50% PEG.

PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization.

생화학적/생리학적 작용

Polyethylene glycol (PEG) is used in detergents, emulsifying agents, soaps, metal costing process, pharmaceutical preparations and cosmetics. PEG is commonly used as a lubricant. It′s application is also observed in chromatographic stationary phases. PEG prevents crystal formation in emulsions.

포장

Packaged in sealed ampules under nitrogen.

기타 정보

Contains 50% (w/v) polyethylene glycol (Av. Mol. Wt. 1450) in DPBS without calcium.

재구성

Solution is ready-to-use. If a less concentrated solution is desired, dilute with sterile DPBS without calcium (D5773). Some precipitate may appear after being exposed to cooler, but should disappear as the solution warms. Solution may be frozen if desired but should first be aliquotted to avoid repeated freeze/thaw cycles.

법적 정보

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

212.0 °F - closed cup

Flash Point (°C)

100 °C - closed cup

개인 보호 장비

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


시험 성적서(COA)

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Construction of two whole genome radiation hybrid panels for dromedary (Camelus dromedarius): 5000 RAD and 15000 RAD
Perelman PL, et al.
Scientific reports, 8(1), 1982-1982 (2018)
Retinol encapsulated into amorphous Ca2+ polyphosphate nanospheres acts synergistically in MC3T3-E1 cells
Muller WEG, et al.
European Journal of Pharmaceutics and Biopharmaceutics, 93(2), 214-223 (2015)
Development of Monoclonal Antibodies against CMP-N-Acetylneuraminate-beta-galactosamide-alpha-2, 3-sialyltransferase 1 (ST3Gal-I) Recombinant Protein Expressed in E. coli
Gupta AK, et al.
Biochemistry Research International, 2015(1), 1982-1982 (2015)

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