추천 제품
항체 생산 유형
primary antibodies
Quality Level
material
polystyrene
클론
monoclonal
유통기한
Unopened plates are stable for 2 years. Once opened they are stable for 2 weeks.
기술
ELISA: suitable
동형
IgG1
용량
100-300 ng/well
저장 온도
2-8°C
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일반 설명
The Anti-FLAG® HS, M2 Coated Plates are designed to ensure a higher binding capacity than other plates. This is due to the orientation of the Anti-FLAG® M2 mouse monoclonal IgG1 antibody on the plates. The antibody is covalently linked to the surface of a microtiter plate via the Fc portion of the antibody.
애플리케이션
ANTI-FLAG® High Sensitivity, M2 coated 96-well plates is used for Enzyme-linked immunosorbent assays. A convenient, ready to use, platform for the capture and detection of FLAG® fusion proteins. The ANTI-FLAG® M2 antibody is covalently attached to the surface through the Fc portion and can detect 1 ng FLAG fusion protein/well with a capacity of up to 300ng/well. Suitable for screening for expression, study of protein:protein interactions and ELISA assays. Manufactured under ISO 9002 in Sigma′s GMP facility, ANTI-FLAG®P2983 high sensitivity M2 coated multiwell plates utilize a flat bottom, polystyrene baseplate.
Learn more product details in our FLAG® application portal.
Learn more product details in our FLAG® application portal.
저장 및 안정성
Once plates are opened, they should be stored with desiccant at 2- 8 °C and used within two weeks.
기타 정보
Components:
The plate is supplied as a 96-well microtiter plate with clear sides and bottom.
Coating:
ANTI-FLAG® M2 mouse monoclonal antibody, IgG1, is coated at a reaction volume of 200 ml/well.
Blocking:
The wells are pre-blocked for convenience at 275 to 300 ml/well with a complex solution containing bovine serum albumin.
Specificity:
The plates are specific for the FLAG epitope regardless of its placement in the fusion protein: amino-terminal, Met-amino terminal, carboxy terminal or internal. Binding of the epitope is not Ca2+ dependent.
Sensitivity:
Detection of 1 ng/well of a control fusion protein was observed in an ELISA format with p-Nitrophenyl Phosphate (pNPP) as a substrate.
Capacity:
Capture of 100 to 300 ng/well of a FLAG fusion protein has been demonstrated.
The plate is supplied as a 96-well microtiter plate with clear sides and bottom.
Coating:
ANTI-FLAG® M2 mouse monoclonal antibody, IgG1, is coated at a reaction volume of 200 ml/well.
Blocking:
The wells are pre-blocked for convenience at 275 to 300 ml/well with a complex solution containing bovine serum albumin.
Specificity:
The plates are specific for the FLAG epitope regardless of its placement in the fusion protein: amino-terminal, Met-amino terminal, carboxy terminal or internal. Binding of the epitope is not Ca2+ dependent.
Sensitivity:
Detection of 1 ng/well of a control fusion protein was observed in an ELISA format with p-Nitrophenyl Phosphate (pNPP) as a substrate.
Capacity:
Capture of 100 to 300 ng/well of a FLAG fusion protein has been demonstrated.
법적 정보
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
F-127 is a registered trademark of BASF SE
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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관련 콘텐츠
Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.
Protein expression technologies for expressing recombinant proteins in E. coli, insect, yeast, and mammalian expression systems for fundamental research and the support of therapeutics and vaccine production.
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