P1588

Placenta Growth Factor human

≥95% (SDS-PAGE), recombinant, expressed in E. coli, powder, suitable for cell culture

• 동의어(들): PGF, PlGF
• MDL number: MFCD00677685
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• product name: Placenta Growth Factor human, PlGF, recombinant, expressed in E. coli, powder, suitable for cell culture
• 생물학적 소스: human
• Quality Level: 200
• 재조합: expressed in E. coli
• 분석: ≥95% (SDS-PAGE)
• 형태: powder
• 품질: endotoxin tested
• 분자량: 29 kDa
• 포장: pkg of 10 and 50 μg
• 저장 조건: avoid repeated freeze/thaw cycles
• 기술: cell culture | mammalian: suitable
• 불순물: <1.0 EU/μg
• 배송 상태: dry ice
• 저장 온도: −70°C

설명

생화학적/생리학적 작용

Placenta growth factor (PlGF) is expressed in the placenta and somewhat less in the heart, lung and thyroid gland. Placentally expressed PlGF may act as an autocrine on trophoblasts, which express both PlGF and its receptor (VEGFR-1). Since these cells also make VEGF-A, natural heterodimers (PlGF/VEGF-A) have also been detected. Two alternatively spliced isoforms of PlGF have been identified.

물리적 형태

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 50 μg bovine serum albumin per 1 μg of cytokine.

제조 메모

Recombinant human PlGF is lyophilized from a 0.2 micron filtered solution of 30% CH3CN and 0.1% TFA (pH 7.4) containing 50 microg of bovine serum albumin per 1 microg of cytokine.

재구성

Reconstitute with sterile PBS containing at least 0.1% HSA or BSA. The rhP/GF concentration should be no less than 10 μg/mL.

분석 메모

The biological activity is measured by the ability to bind rhFlt-1/Fc in a functional ELISA. Immobilized rhFlt-1/Fc at 2 μg/mL (100 μL/well) can bind rhP/GF with a linear range at 0.1 - 5 ng/mL.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)