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Key Documents

P1291

Sigma-Aldrich

Peroxidase Anti-Peroxidase Soluble Complex antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

동의어(들):

Antiperoxidase, Pap Antibody, Peroxidase Anti Peroxidase, Peroxidase Anti Peroxidase - Peroxidase Anti-Peroxidase Soluble Complex antibody produced in rabbit, PAP

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.46

생물학적 소스

rabbit

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

형태

buffered aqueous solution

기술

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200

배송 상태

dry ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

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특이성

By immunoelectrophoresis (IEP), a single arc of precipitation is observed when the product (prior to the addition of BSA) is electrophoresed and then allowed to diffuse against anti-rabbit whole serum or anti-rabbit IgG. No reaction is observed against free horseradish peroxidase.

애플리케이션

Peroxidase Anti-Peroxidase Soluble Complex antibody produced in rabbit has been used in:
  • immunoblotting
  • to detect the expression of TAP-tagged proteins
  • immunoprecipitation
  • immunohistochemical staining

Peroxidase Anti-Peroxidase Soluble Complex antibody produced in rabbit was used in immunoblotting of yeast whole cell extracts.
Sigma PAP is assayed by immunoelectrophoresis, Ouchterlony double diffusion, and immunohistochemical staining techniques to ensure purity and functional reactivity. It is recommended that the optimal working dilution be determined in the specific assay system.
Sigma Peroxidase Anti-Peroxidase Soluble Complex antibody produced in rabbit is assayed by immunoelectrophoresis, Ouchterlony double diffusion, and immunohistochemical staining techniques to ensure purity and functional reactivity. It is recommended that the optimal working dilution be determined in the specific assay system.

생화학적/생리학적 작용

Peroxidase:anti-peroxidase (PAP) immune complex is inexpensive immunoglobulin ligand. It is widely used for the detection of fragment crystallisable (Fc) receptor binding and fragment antigen binding (Fab) regions.
The Peroxidase Anti-Peroxidase (PAP) soluble complex is prepared by a modification of the method of Sternberger using specific polyclonal or monoclonal antibodies to horseradish peroxidase and highly purified enzyme. Since the enzyme is not covalently coupled to the antibody, the soluble complex retains its enzyme and antibody reactivity with distinct advantages over enzyme-conjugated antibodies.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

저장 및 안정성

Store at –20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots at –20 °C. Repeated freezing and thawing or storage in "frost-free" freezers, is notrecommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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픽토그램

Exclamation mark

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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