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Merck
모든 사진(1)

주요 문서

N8773

Sigma-Aldrich

Anti-Nerve Growth Factor-β antibody produced in goat

affinity isolated antibody, lyophilized powder

동의어(들):

Anti-NGF-β

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About This Item

MDL number:
UNSPSC 코드:
51111800
NACRES:
NA.41

생물학적 소스

goat

Quality Level

결합

unconjugated

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

클론

polyclonal

양식

lyophilized powder

종 반응성

rat

기술

capture ELISA: 0.2-0.8 μg/mL
immunohistochemistry: 5-15 μg/mL
neutralization: 0.02-0.1 μg/mL
western blot: 0.1-0.2 μg/mL

UniProt 수납 번호

저장 온도

−20°C

타겟 번역 후 변형

unmodified

유전자 정보

rat ... Ngf(310738)

일반 설명

Nerve Growth factor (NGF) is a growth factor that is essential for the development and maintenance of the nervous system. NGF is composed of three non-covalently linked α, β and γ subunits. The β subunit is responsible for the biological functions of NGF. Nerve growth factor (NGF) binds to either to NGF receptor (NGFR) or the Trk receptor and can elicit biological responses through each of the receptors independently. NGF is important in the survival and differentiation of neurons and neural crest-derived cells during embryonic and early postnatal development and maintenance of peripheral neurons. NGF is also implicated in inflammation and allergic reactions . Anti-Nerve Growth Factor-β antibody recognizes rat NGF-β. The antibody shows less than 1% cross reactivity with recombinant human β-NGF.
in the presence of 10.0μg/ml of recombinat rat β-NGF using the human erythroleukemic TF-1 cell line.

면역원

recombinant rat β-nerve growth factor expressed in Sf 21 cells.

애플리케이션

Anti-NGF-β antibody may be used for neutralization assays; the ND50 is ~0.02-0.1 μg/ml. For immunoblotting the recommended antibody concentration is 0.1-0.2 μg/ml. The antibody is suitable for immunohistochemistry at a working concentration of 5-15 μg/ml. For ELISA, antibody concentration of 0.2-0.8 μg/ml may be used. Antibody dilution of 1:500 was used for immunoblotting of extracts of rat nerve cells.

물리적 형태

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서 라이브러리 방문

Margherita Maranesi et al.
Biology of reproduction, 98(5), 634-643 (2018-02-14)
To investigate the ovulatory mechanisms triggered by raw semen (RS) in rabbits, we examined the expression of nerve growth factor (NGF)-a supposed ovulation-inducing factor (OIF)-and cognate receptors in anterior pituitary, ovary, and cervix as well as plasma NGF and luteinizing
Bopaiah P Cheppudira et al.
BMC anesthesiology, 16(1), 73-73 (2016-09-07)
Nerve growth factor (NGF) is known to induce inflammation and pain; however its role in opioid-induced tolerance has not been studied. This study investigated the effects of an anti-NGF neutralizing antibody on the development of tolerance following chronic morphine treatment
Uros Kovacic et al.
Neurobiology of aging, 31(2), 339-350 (2008-05-24)
Sprouting of uninjured nociceptive axons was examined in young adult, middle aged and aged rats. Axon sprouting from the spared sural nerve, both into adjacent denervated skin and into end-to-side coapted nerve graft, was significantly higher in young rats than
Ana Sanchez-Rodriguez et al.
Theriogenology, 126, 206-213 (2018-12-24)
β-Nerve Growth Factor (β-NGF) is a neurotrophin which acts through its receptors TrkA and p75, performing important actions in male reproductive physiology and its presence in seminal plasma (SP) has been related to male fertility. The aim of the present
Nerve growth factor and the physiology of pain: lessons from congenital insensitivity to pain with anhidrosis
Indo Y
Clinical Genetics, 82, 341-350 (2010)

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