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Key Documents

추가 자료

N3254

Sigma-Aldrich

4-Nitrophenyl phosphate di(tris) salt

phosphatase substrate, ≥97% (enzymatic), powder

동의어(들):

4-Nitrophenyl phosphate bis(tris) salt

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모든 사진(1)

About This Item

Linear Formula:
(C6 H4 N O6 P)( C4 H12 N O3)2
CAS Number:
68189-42-4
Molecular Weight:
461.36
Beilstein:
1578142
EC Number:
269-198-6
MDL number:
MFCD00037009
UNSPSC 코드:
12352204
PubChem Substance ID:
24897633
NACRES:
NA.83

product name

4-Nitrophenyl phosphate di(tris) salt, phosphatase substrate

분석

≥97% (enzymatic)

형태

powder

solubility

water: 50 mg/mL, clear, colorless to light yellow

저장 온도

−20°C

SMILES string

NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O

InChI

1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2

InChI key

XXAXKCWOTRABOW-UHFFFAOYSA-N

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관련 카테고리

애플리케이션

4-Nitrophenyl phosphate di(tris) salt has been used:
  • as a substrate to quantify tartrate-resistant acid phosphatase (TRAP) activity in cell lysates
  • to determine phosphatase activity using native polyacrylamide gel electrophoresis (PAGE)
  • for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA)

생화학적/생리학적 작용

p-Nitrophenyl Phosphate (pNPP) is a preferred substrate for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures, because of its high sensitivity. This substrate produces a soluble yellow end-product and can be read spectrophotometrically at 405 nm.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)

시험 성적서(COA)

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