N1531

Denaturation Solution

for Northern and Southern blotting

• 동의어(들): Denaturation Solution For Blotting Technique, Denaturation Solution for Neutral Southern Transfer
• UNSPSC 코드: 12161700
• NACRES: NA.25

속성

• Grade: Molecular Biology; for molecular biology
• 양식: solution
• 저장 온도: room temp

설명

일반 설명

Sigma′s denaturing solution is a strong base that denatures double-stranded DNA so that it can be efficiently transferred to nylon membrane and subsequently hybridized to labeled probe. When used with a depurination solution, treatment results in the hydrolysis of the phosphodiester backbone at the sites of depurination, allowing for more efficient transfer of large DNA in neutral Southern transfers.

애플리케이션

Denaturation Solution is suitable for denaturing dsDNA before the transfer to membranes during Northern and Southern blotting procedures.

성분

The denaturing solutions is composed of 0.5N NaOH, and 1.5M NaCl.

기타 정보

0.5 N NaOH, 1.5 M NaCl

안전성 정보

• 픽토그램: GHS05
• 신호어: Danger
• 유해 및 위험 성명서: H290,H314
• 예방조치 성명서: P234 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363
• Hazard Classifications: Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B
• Storage Class Code: 8B - Non-combustible corrosive hazardous materials
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable