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주요 문서

MSANGERV

Sigma-Aldrich

Sanger Arrayed Whole Genome Lentiviral CRISPR Library

Mouse, Virus Format

동의어(들):

Arrayed CRISPR library, Sanger CRISPR library

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About This Item

UNSPSC 코드:
41105904

Quality Level

200

포장

pkg of 10 μL (384-well plate)

농도

1x106  VP/ml (via p24 assay)

응용 분야

CRISPR

배송 상태

dry ice

저장 온도

−70°C

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관련 카테고리

일반 설명

Two leaders in genome editing, Sigma-Aldrich and the Wellcome Trust Sanger Institute, have joined forces to make the first ever arrayed lentiviral CRISPR knockout libraries. Based upon validated techniques published in the literature, the Sanger CRISPR libraries will put your lab at the forefront of the race to make the next big discovery.

애플리케이션

Functional Genomics/Screening /Target Validation

특징 및 장점

  • Vector: U6-gRNA/PGK-Puro-2A-BFP (gRNA only)
  • Simplify the workflow with puromycin selection
  • Illuminate CRISPR-expressing cells with BFP

Additional Features
  • Better, not bigger: Two optimized clones per mouse gene reduces the time, cost, and scale of screening experiments
  • Ready-to-screen: Clones are arrayed in a robotics-friendly 384-well format for high throughput screening
  • Collaborative: Real-time, library validation continues

For detailed information on the Sanger library, click here

포장

384 well plates

성분

Lentivirus Transduction Particles of gRNA-only lenti-based vectors. 2 knockout clones for every human protein-coding gene. Nearly 40,000 sequence confirmed clones


Request a Quote or More Information

물리적 형태

10μl of lentivirus at ≥106 particles/ml in ~120×384 well plates

기타 정보

This product is for R&D use only, not for drug, household, or other uses. Lentivirus manufacturing is achieved by using 2nd generation packaging plasmids on a 3rd generation lenti-based vector. Though the lentiviral transduction particles produced are replication incompetent, it is recommended that they be treated as Risk Group Level 2 (RGL-2) organisms in laboratory handling. Follow all published RGL-2 guidelines for laboratory handling and waste decontamination.

추천 제품

Additional libraries are available:
Human, Glycerol Format
Mouse, Glycerol Format
Human, Virus Format

법적 정보

CRISPR Use License Agreement

Lentiviral License Agreement

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Enhancing the genome editing toolbox: genome wide CRISPR arrayed libraries.
Metzakopian, E. et al
Scientific Reports, 7 (2017)

문서

Successful Transduction Using Lentivirus

Successful targeting relies on optimizing key sensitive steps in the process, including lentiviral transduction. Below are some helpful handling and titration tips from our R&D lentiviral experts.

프로토콜

Sanger Sequencing Steps & Method

Learn about Sanger Sequencing steps or the chain termination method and how DNA sequencing works and how to read Sanger Sequencing results accurately for your research.

Sanger 염기서열분석 단계 및 기법

Sanger 염기서열분석의 단계 또는 사슬종료법에 대해 알아보고 DNA 염기서열분석이 작동하는 방법, 연구 시 Sanger 염기서열분석 결과를 정확하게 판독하는 방법에 대해서 자세히 알아보십시오.

FACS Titration of Lentivirus Protocol

FACS (Fluorescence-Activated Cell Sorting) provides a method for sorting a mixed population of cells into two or more groups, one cell at a time, based on the specific light scattering and fluorescence of each cell. This method provides fast, objective, and quantitative recording of fluorescent signals from individual cells.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.