MAK380

LDH-Cytotoxicity Assay Kit II

sufficient for 500 colorimetric tests

• UNSPSC 코드: 12161503

속성

• 검출 방법: colorimetric
• 저장 온도: −20°C

설명

일반 설명

The new LDH Cytotoxicity Assay Kit, MAK529, is now available! Cell death or cytotoxicity is classically evaluated by the quantification of plasma membrane damage. Lactate dehydrogenase (LDH) is a stable enzyme, present in all cell types, and rapidly released into the cell culture medium upon damage of the plasma membrane. LDH, therefore, is the most widely used marker in cytotoxicity study.

특징 및 장점

The LDH-Cytotoxicity Assay Kit II:

• Is a non-radioactive assay.
• Simple one-step procedure.

적합성

The kit is suitable for the determination of Lactate dehydrogenase (LDH) activity in cells.

원리

The LDH Cytotoxicity Assay Kit II utilizes the advanced WST (Water Soluble Tetrazolium) reagent for a fast and more sensitive detection of LDH released from the damaged cells. The assay utilizes an enzymatic coupling reaction: LDH oxidizes lactate to generate NADH, which then reacts with WST to generate yellow color at A450 nm. The intensity of the generated color correlates directly with the cell number lysed.

안전성 정보

• 픽토그램: GHS05
• 신호어: Danger
• 유해 및 위험 성명서: H314
• 예방조치 성명서: P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363 - P405
• Hazard Classifications: Eye Dam. 1 - Skin Corr. 1B
• Storage Class Code: 8A - Combustible corrosive hazardous materials