MAK317

Sorbitol Dehydrogenase Assay Kit

sufficient for 100 colorimetric tests

• UNSPSC 코드: 12161503
• NACRES: NA.84

속성

• 검출 방법: colorimetric
• 관련 질환(들): endocrinological disorders, diabetes; gastrointestinal diseases
• 저장 온도: −20°C

설명

일반 설명

Sorbitol Dehydrogenase (SDH) is an enzyme that catalyzes the interconversion of sorbitol and fructose. Elevated blood serum SDH levels indicate liver damage. Thus, SDH plays an important role in the diagnosis of liver disease, especially in combination with aminotransferases. SDH levels are also measured to evaluate diabetic complications such as proliferative diabetic retinopathy.

Sorbitol Dehydrogenase (SDH) is an enzyme that catalyzes the interconversion of sorbitol and fructose. Elevated blood serum SDH levels indicate liver damage; thus, SDH plays an important role in the diagnosis of liver disease, especially in combination with aminotransferases. SDH levels are also measured to evaluate diabetic complications such as proliferative diabetic retinopathy.

특징 및 장점

Compatible with high-throughput handling systems.

적합성

Suitable for determining sorbitol dehydrogenase (SDH) activity in a number of biological samples such as plasma, serum, urine, tissue, and culture media.

원리

Sorbitol dehydrogenase assay is a non-radioactive, colorimetric assay. It is based on the reduction of the tetrazolium salt MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide) in a NADH-coupled enzymatic reaction. The reduced form of MTT exhibits an absorption maximum at 565 nm and the increase in absorbance at 565 nm is directly proportional to the enzyme activity. This assay is based on a kinetic reaction. Sorbitol dehydrogenase (SDH) activity can be determined in a number of biological samples (e.g., plasma, serum, urine, tissue, and culture media).

안전성 정보

• 픽토그램: GHS02,GHS05,GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H226 - H290 - H315 - H319 - H336
• 예방조치 성명서: P210 - P233 - P234 - P240 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 2
• Flash Point (°F): 75.2 °F - closed cup
• Flash Point (°C): 24 °C - closed cup