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Merck
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Key Documents

MAK122

Sigma-Aldrich

Phospholipid Assay Kit

sufficient for 100 colorimetric or fluorometric tests

동의어(들):

Phospholipid Quantification Kit

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About This Item

UNSPSC 코드:
12161503
NACRES:
NA.84

사용

sufficient for 100 colorimetric or fluorometric tests

응용 분야

cosmetics
food and beverages
pharmaceutical

검출 방법

colorimetric
fluorometric

배송 상태

dry ice

저장 온도

−20°C

일반 설명

Phospholipids are a class of lipids which constitute a major component of cell membranes and play important roles in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline.

In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2-specific dye. This results in a colorimetric (570 nm)/ fluorometric (λex = 530/λem = 585 nm) product directly proportional to the phospholipid concentration in the sample. The range of linear detection is 3 - 200 μM for colorimetric assays and 0.6 - 20 μM for fluorometric assays.

애플리케이션

Phospholipid assay kit has been used in biochemical analysis and phospholipid quantitation.

특징 및 장점

Compatible with high-throughput handling systems.

적합성

Suitable for the detection of phospholipids in biological samples

원리

In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2-specific dye. This results in a colorimetric (570 nm)/ fluorometric (λex = 530/λem = 585 nm) product directly proportional to the phospholipid concentration in the sample. The range of linear detection is 3 - 200 μM for colorimetric assays and 0.6 - 20 μM for fluorometric assays.

픽토그램

Health hazard

신호어

Danger

유해 및 위험 성명서

예방조치 성명서

Hazard Classifications

Aquatic Chronic 3 - Resp. Sens. 1

Storage Class Code

10 - Combustible liquids


시험 성적서(COA)

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