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사용
sufficient for 100 fluorometric tests
검출 방법
fluorometric
관련 질환(들)
endocrinological disorders, diabetes; cancer
저장 온도
−20°C
일반 설명
The new Glucose Uptake Assay Kit, MAK489, is now available! Glucose is the primary source of energy for most cells.Transport of glucose across the plasma membrane is the first rate limiting step in glucose metabolism. Glucose uptake is facilitated by the GLUT family of transporter proteins, whose expression and activity are regulated by multiple mechanisms. Glucose uptake is upregulated in many cancer cells, which exhibit high rates of aerobic glycolysis. Cells exhibiting insulin resistance show diminished glucose uptake in response to insulin stimulation.
애플리케이션
Glucose Uptake Fluorometric Assay Kit has been used to measure the amount of glucose uptake by monocytic myeloid-derived suppressor cells (M-MDSCs).
적합성
Suitable for detecting glucose uptake in adherent or suspension cells cultured in a 96-well microtiter plate.
원리
The Glucose Uptake Fluorometric Assay kit provides a simple and direct procedure for measuring glucose uptake in a variety of cells. Glucose uptake is measured using the glucose analog, 2-deoxyglucose (2-DG), which is taken up by cells and phosphorylated by hexokinase to 2-DG6P. 2-DG6P cannot be further metabolized and accumulates in cells, directly proportional to the glucose uptake by cells. In this assay, 2-DG uptake is determined by a coupled enzymatic assay in which the 2-DG6P is oxidized, resulting in the generation of NADPH, which reacts with the probe to generate a fluorometric (λex = 535/λem = 587 nm) product, proportional to the 2-DG taken up by the cell.
유해 및 위험 성명서
예방조치 성명서
Hazard Classifications
Aquatic Chronic 3
Storage Class Code
10 - Combustible liquids
Flash Point (°F)
188.6 °F - closed cup
Flash Point (°C)
87 °C - closed cup
가장 최신 버전 중 하나를 선택하세요:
시험 성적서(COA)
Lot/Batch Number
Tingting Wu et al.
Scientific reports, 6, 20250-20250 (2016-02-03)
CD11b(+) Gr1(+) myeloid-derived suppressor cells (MDSCs) play critical roles in controlling the processes of tumors, infections, autoimmunity and graft rejection. Immunosuppressive drug rapamycin (RPM), targeting on the key cellular metabolism molecule mTOR, is currently used in clinics to treat patients
Mechanisms for insulin resistance: common threads and missing links.
Samuel V T and Gerald I S
Cell, 148(5), 852-871 (2012)
Chapter Twenty-Two Mechanisms and Methods in Glucose Metabolism and Cell Death.
Zhao Y, et al.
Methods in Enzymology, 442, 439-457 (2008)
Yang Zhao et al.
Blood, 131(14), 1587-1599 (2018-02-22)
Monocytes and macrophages play a key role in defending pathogens, removing the dead cells or cell debris, and wound healing. The mammalian target of rapamycin (mTOR) inhibitor rapamycin (RPM) is widely used in clinics to treat patients with organ transplantation
Norio Yamamoto et al.
Analytical biochemistry, 375(2), 397-399 (2008-02-12)
To determine 2-deoxy-D-glucose (2DG) and 2-deoxy-D-glucose 6-phosphate (DG6P) in mouse tissue after injection of 2DG, we have developed a novel assay. This assay is a simple procedure involving incubation of samples with four independent, single reaction mixtures followed by measurement
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