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Merck
모든 사진(2)

주요 문서

MAK075

Sigma-Aldrich

Ascorbic Acid Assay Kit II

sufficient for 100 colorimetric tests

동의어(들):

FRASC Assay, Ferric Reducing/Antioxidant and Ascorbic Acid

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About This Item

UNSPSC 코드:
12161503
NACRES:
NA.84

사용

sufficient for 100 colorimetric tests

검출 방법

colorimetric

저장 온도

−20°C

일반 설명

The new Ascorbic Acid Assay Kit, MAK505, is now available! Ascorbic Acid, also known as Vitamin C, is a six-carbon lactone produced by plants and some animal species but not by humans and other primates. Ascorbic acid functions as an enzymatic cofactor for multiple enzymes, serving as an electron donor for monooxygenases and dioxygenases. Ascorbic acid also functions as a powerful antioxidant, particularly in regards to reactive oxygen species.

애플리케이션

Ascorbic Acid Assay Kit II has been used to determine ascorbic acid concentration in vitreous sample from the eye.

적합성

Suitable for use with cell and tissue culture supernatants as well as serum, plasma, urine, and other biological fluids.

원리

In this assay, ascorbic acid concentration is determined using the Ferric Reducing/Antioxidant and Ascorbic Acid (FRASC) assay. Fe3+ is reduced to Fe2+ by antioxidants present in the sample, which results in a colorimetric (593 nm) product. The addition of ascorbate oxidase to parallel samples oxidizes any ascorbic acid present allowing for the measurement of the ascorbic acid concentration.

Storage Class Code

10 - Combustible liquids


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문서 라이브러리 방문

Karthik Murali et al.
PloS one, 11(3), e0149961-e0149961 (2016-03-02)
We investigated the spatial variation of vitreous oxygen consumption in enucleated porcine eyes. A custom made oxygen source was fabricated that could be localized to either the mid or posterior vitreous cavity and steady state vitreous oxygen tension was measured
Li-Zhen Zheng et al.
Biomaterials, 238, 119828-119828 (2020-02-12)
Magnesium (Mg)-based biometal attracts clinical applications due to its biodegradability and beneficial biological effects on tissue regeneration, especially in orthopaedics, yet the underlying anabolic mechanisms in relevant clinical disorders are lacking. The present study investigated the effect of magnesium (Mg)

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