추천 제품
사용
sufficient for 100 colorimetric tests
검출 방법
colorimetric
관련 질환(들)
cancer
저장 온도
−20°C
유전자 정보
human ... IDH1(3417) , IDH2(3418) , IDH3B(3420) , IDH3G(3421)
mouse ... IDH1(15926) , IDH2(269951) , IDH3B(170718) , IDH3G(15929)
rat ... IDH1(24479) , IDH2(361596) , IDH3B(94173) , IDH3G(25179)
일반 설명
Isocitrate dehydrogenase (IDH) catalyzes the conversion of isocitrate to α-ketoglutarate. In eukaryotes, there are three isozymes of IDH, the mitochondrial IDH2 and IDH3, and the cytoplasmic/ peroxisomal IDH1. All three IDH family members require the presence of a divalent cation (Mg2+ or Mn2+) and either the electron-accepting cofactor NADP+ (IDH1 and IDH2) or NAD+ (IDH3) for their enzymatic activity. IDH1 and IDH2 mutations resulting in neomorphic enzymatic activity are found in certain cancers such as glioblastoma, acute myeloid leukemia, and colon cancer. This neoactivity shows a change in the substrate specificity resulting in the conversion of α-ketoglutarate to 2-hydroxyglutarate. Mutations in IDH family members are also associated with Ollier disease and Maffucci syndrome.
애플리케이션
IDH Activity Assay Kit has been used to determine the activity of isocitrate dehydrogenase in samples.
특징 및 장점
Compatible with high-throughput handling systems.
적합성
Suitable for the measurement of isocitrate dehydrogenase (IDH) (NAD+ - dependent, NADP+ -dependent or both IDHs) activity in biological samples including tissue, cells and serum
원리
The Isocitrate Dehydrogenase Activity Assay kit provides a simple and direct procedure for measuring NADP+-dependent, NAD+-dependent, or both NADP+ and NAD+-dependent IDH activity in a variety of samples. IDH activity is determined using isocitrate as the substrate in an enzyme reaction, which results in a colorimetric (450 nm) product proportional to the enzymatic activity present. One unit of IDH is the amount of enzyme that will generate 1.0 μmole of NADH or NADP per minute at pH 8.0 at 37 °C.
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B
Storage Class Code
8A - Combustible corrosive hazardous materials
가장 최신 버전 중 하나를 선택하세요:
시험 성적서(COA)
Lot/Batch Number
Trilobatin Protects Against Oxidative Injury in Neuronal PC12 Cells Through Regulating Mitochondrial ROS Homeostasis Mediated by AMPK/Nrf2/Sirt3 Signaling Pathway.
Gao J, et al.
Frontiers in Molecular Neuroscience, 11, 267-267 (2018)
Ye Kuang et al.
Cancer medicine, 7(4), 1416-1429 (2018-03-14)
Most cancer cells perform glycolysis despite having sufficient oxygen. The specific metabolic pathways of cancer cells have become the focus of cancer treatment. Recently, accumulating evidence indicates oxidative phosphorylation (OXPHOS) and glycolysis can be regulated with each other. Thus, we
Enrique Chávez et al.
The Journal of pharmacology and experimental therapeutics, 361(2), 292-302 (2017-02-18)
Background: Mitochondrion is an important metabolic and energetic organelle that regulates several cellular processes. Mitochondrial dysfunction has been related to liver diseases including hepatocellular carcinoma. As a result, the energetic demand is not properly supplied and mitochondrial morphologic changes have
Subhamoy Dasgupta et al.
The Journal of clinical investigation, 125(3), 1174-1188 (2015-02-11)
Metabolic pathway reprogramming is a hallmark of cancer cell growth and survival and supports the anabolic and energetic demands of these rapidly dividing cells. The underlying regulators of the tumor metabolic program are not completely understood; however, these factors have
프로토콜
We describe here a rapid and sensitive method to separate and measure D-2-OHG and L-2-OHG enantiomers using high-resolution mass spectrometry (HRMS) detection.
Chromatograms
application for HPLC자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
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