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Merck
모든 사진(3)

주요 문서

MAK001

Sigma-Aldrich

Alanine Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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모든 사진(3)

About This Item

UNSPSC 코드:
12161503
NACRES:
NA.84

사용

sufficient for 100 colorimetric or fluorometric tests

검출 방법

colorimetric
fluorometric

저장 온도

−20°C

일반 설명

Alanine is a nonessential amino acid that plays a key role in the glucose-alanine cycle between muscle tissue and the liver. In amino acid-degrading tissues such as muscle, amino groups are pooled as glutamate by transamination reactions. The amino group of glutamate is transferred to pyruvate via alanine aminotransferase, forming alanine and α-ketoglutarate. The alanine is passed into the blood and transported to the liver. This reaction is reversed in the liver where pyruvate can be used in gluconeogenesis to form glucose, which may return to other tissues through the circulatory system. Increased alanine levels correlate with higher blood pressure, energy intake, cholesterol levels, and body mass index.

애플리케이션

Alanine Assay Kit has been used to determine the intracellular alanine pool.

적합성

Suitable for alanine detection in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples.

원리

In this kit, alanine concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the alanine present. Typical detection ranges for this kit are 2-10 nmole (colorimetric) and 0.2-1 nmole (fluorometric).

교체됨

제품 번호
설명
가격

픽토그램

Health hazard
GHS08

신호어

Danger

유해 및 위험 성명서

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

Flash Point (°F)

188.6 °F - closed cup

Flash Point (°C)

87 °C - closed cup

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시험 성적서(COA)

Lot/Batch Number
7K22K06520
7F14K06520
7D07K06520
6E21K06520
5K28K06520

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COA 검색

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Mauro D P.
Amino Acids and Proteins for the Athlete: The Anabolic Edge (1997)
The complex role of branched chain amino acids in diabetes and cancer.
O'Connell T M.
Metabolites, 3(4), 931-945 (2013)
GABA transaminases from Saccharomyces cerevisiae and Arabidopsis thaliana complement function in cytosol and mitochondria.
Cao J, et al.
Yeast, 30(7), 279-289 (2013)
Berg J M, et al.
Biochemistry (5th Edition) (2002)
Dmitry Frank et al.
Neuropharmacology, 155, 173-184 (2019-06-04)
Post-stroke depression (PSD) is a common and serious complication following stroke. Both stroke and depression have independently been associated with pathologically elevated glutamate levels in the brain's extra-cerebral fluid (ECF). Here we evaluate an alternative therapeutic approach to PSD with
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문서

Glutamine Metabolism is Dysregulated in Many Cancer Cells

Sigma-Aldrich presents an article about how proliferatively active cells require both a source of carbon and of nitrogen for the synthesis of macromolecules. Although a large proportion of tumor cells utilize aerobic glycolysis and shunt metabolites away from mitochondrial oxidative phosphorylation, many tumor cells exhibit increased mitochondrial activity.

프로토콜

Enzymatic Method for Determining Alanine (Alanine Assay)

Assay protocol for the colorimetric/fluorometric detection of Alanine in biological samples using the alanine assay kit.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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