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M8042

Sigma-Aldrich

Minimum Essential Medium Eagle

Alpha Modification, with nucleosides and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

동의어(들):

EMEM, MEM

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About This Item

UNSPSC 코드:
12352207
NACRES:
NA.75

product name

Minimum Essential Medium Eagle, Alpha Modification, with ribonucleosides, deoxyribonucleosides and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

무균

sterile-filtered

형태

liquid

기술

cell culture | mammalian: suitable

불순물

endotoxin, tested

성분

Hanks’ salts (2% CO2): no
glucose: yes
sodium pyruvate: yes
phenol red: yes
HEPES: no
L-glutamine: no
stable glutamine: no
Earle’s salts (5% CO2): yes
NaHCO3: yes

배송 상태

ambient

저장 온도

2-8°C

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.This is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins. Often used in cytogenetic analysis when supplemented with fetal bovine serum (FBS).

애플리케이션

Minimum Essential Medium Eagle has been used for the isolation of mesenchymal stem cell, adipose-derived stem cell, and osteoblast.
Minimum Essential Medium Eagle is used for primary osteoblast cultures and for isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood.

생화학적/생리학적 작용

MEM usually autoclaved with 0.5% bactopeptone at 15 psi for 20 min, cooled and then neutralized with NaHCO3, consistently supported good cell growth. A similar result was obtained when the MEM and bactopeptone were autoclaved separately. The cells grow, initially, as a monolayer and subsequently become a stationary suspension.

재구성

Supplement with 0.292 g/L L-glutamine

또한 이 제품과 함께 일반적으로 구입

제품 번호
설명
가격

보충제

제품 번호
설명
가격

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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Chlorogenic acid (CGA) is a primary phenolic component of coffee and (-)-epigallocatechin gallate (EGCG) is a primary flavonoid component of green tea, both of which have been documented to possess beneficial health properties. A previous study by the present authors
A previously functional tetracycline-regulated transactivator fails to target gene expression to the bone
Schmidt E and Eriksson M
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The tetracycline-controlled transactivator system is a powerful tool to control gene expression in vitro and to generate consistent and conditional transgenic in vivo model organisms. It has been widely used to study gene function and to explore pathological mechanisms involved
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The growth of L-60TM cells (a suspension culture adapted L-cell) on media composed of MEM (minimum essential medium (Eagle)) and bactopeptone autoclaved together or separately under a variety of conditions has veen determined. It has been found that MEM autoclaved
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