추천 제품
일반 설명
IgG antibodies are mainly responsible for the facilitation of humoral immune responses such as complement fixation, placental transport, and phagocytosis .
Mouse IgG2b, Kappa is produced in the MOPC 195 tumor line , is a mineral oil-induced plasmacytoma originated and carried intraperitoneally in BALB/c mice.
The MOPC 195 tumor line that produces mouse IgG2b, k is a mineral oil-induced plasmacytoma originated and carried intraperitoneally in BALB/c mice.
The hapten binding specificity of the MOPC 195 line has not been determined. The ascites produced from the MOPC 195 tumor line contains IgG2b, k in addition to normal levels of other mouse immunoglobulins and serum proteins. The ascites fluid is clarified by centrifugation and filtration.
The hapten binding specificity of the MOPC 195 line has not been determined. The ascites produced from the MOPC 195 tumor line contains IgG2b, k in addition to normal levels of other mouse immunoglobulins and serum proteins. The ascites fluid is clarified by centrifugation and filtration.
특이성
Goat anti-guinea pig IgG (whole molecule) -alkaline phosphatase antibody reacts specifically with IgG of guinea pig.
Identity and purity of the antibody is established by immunoelectrophoresis (IEP) and Ouchterlony Double Diffusion (ODD). Electrophoresis of the ascites fluid followed by diffusion versus anti-mouse IgG2b and anti mouse kappa chain results in single arcs of precipitation. By ODD the ascites fluid (containing mouse IgG2b, k) is compared to purified myeloma protein.
면역원
Purified guinea pig IgG
애플리케이션
Anti-guinea pig IgG (whole molecule) −alkaline phosphatase antibody (diluted 1: 30,000 in PBST with 0.2% BSA and 2% PVP) can be used in ELISA. It is also useful in western blotting and immunohistology.
품질
Each vial contains at least 5mg of mouse IgG2b, k myeloma protein, determined by densitometry of electrophoresed ascites fluid.
물리적 형태
lyophilized powder
저장 및 안정성
To one vial of lyophilized powder, add 1 ml of deionized water and an appropriate antimicrobial agent (e.g. sodium azide) to a final concentration of 15 mM. Rotate vial gently until powder dissolves. Prior to reconstitution store the product at 2-8 °C. After reconstitution, the solution may be stored frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage clarify the solution by centrifugation before use
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
E Gustafsson et al.
Biology of reproduction, 51(6), 1173-1180 (1994-12-01)
Mice lacking functional B cells because of a genetic deletion of the mu chain (IgM) gene were used to investigate the role of perinatal and postnatal transfer of maternal IgG in neonatal growth. Our results confirmed that immunoglobulin (Ig)-deficient mice
A V Karasev et al.
Phytopathology, 88(10), 1040-1045 (2008-10-24)
ABSTRACT The beet yellow stunt virus (BYSV) genome contains at least nine open reading frames (ORFs) that code for proteins ranging from 6 to 66 kDa. Based on amino acid sequence comparisons, the coat protein (CP) was previously identified as
Xing-He Weng et al.
The Journal of experimental biology, 206(Pt 13), 2211-2219 (2003-05-29)
The V-type H(+)-ATPase is thought to provide the driving force for transepithelial electrolyte and fluid secretion in Malpighian tubules. To confirm the presence of this proton pump in Malpighian tubules of the yellow fever mosquito Aedes aegypti, we used several
R G Hamilton
Clinical chemistry, 33(10), 1707-1725 (1987-10-01)
Complement activation, cell surface-receptor binding, blocking activity, and possibly placental transfer are among the biologically important functional differences that have been detected between the four human IgG subclasses by use of polyclonal antisera. In 1985, a IUIS/WHO panel of immunologists
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